Sequences from confirmation amplicon


Confirmation sequences for line SALK_030639 and hit At1g76410 are shown.
Line ID SALK_030639
Locus At1g76410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28669421
Primers used for PCR 37vi - LBb3, show primer details
   
Sequence Name 56-K117945-SALK_030639C-37vi
GenBank Accession LN485649 [GenBank]
Sequencing primer gene specific primer, 37vi: 5'-TTCTCCAAGAAGCCAATTCTACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K117945-SALK_030639C-37vi
GCTTCACTCGACCTCGTCCCATCCTTGCGGTTCTTCTTTGTGCACTGACATGTATCATCG
GCTTAATAGCCGTCTCTCGATGCGCTTGGCTCCGCCGTATCGCCTCAAGAAACAGATCGG
ATCAAACTCATCCACCGCCGGTAGCTGCAGCCAACAAAGGATTGAAAAAGAAAGTCCTCC
GGTCGTTACCGAAACTCACCTACTCGCCGGATTCTCCGCCGGCGGAGAAACTCGTGGAGT
GTGCTATCTGTTTAACGGAATTCGCCGCCGGAGACGAGCTTAGGGTGTTGCCGCAGTGTG
GACATGGTTTCCACGTATCGTGTATCGACACGTGGCTTGGATCACACTCTTCTTGTCCTT
CTTGCCGTCAAATCTTGGTGGTTACCAGGTGTCATAAATGTGGCGGGTTACCCGGTAGCT
CAAGTTCAGGACCCGAATCAAAAATGGGAGGATATATTGTGGTGTAAACAAATTGACGCT
TAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCA
CCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCA
AGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 21-K117944-SALK_030639C-R210
GenBank Accession LN484896 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K117944-SALK_030639C-R210
CGTCATTTGTTTACCCACAATATATCCTCCCATTTTTGATTCGGGTCCTGAACTTGAGCT
ACCGGGTAACCCGCCACATTTATGACACCTGGTAACCACCAAGATTTGACGGCAAGAAGG
ACAAGAAGAGTGTGATCCAAGCCACGTGTCGATACACGATACGTGGAAACCATGTCCACA
CTGCGGCAACACCCTAAGCTCGTCTCCGGCGGCGAATTCCGTTAAACAGATAGCACACTC
CACGAGTTTCTCCGCCGGCGGAGAATCCGGCGAGTAGGTGAGTTTCGGTAACGACCGGAG
GACTTTCTTTTTCAATCCTTTGTTGGCTGCAGCTACCGGCGGTGGATGAGTTTGATCCGA
TCTGTTTCTTGAGGCGATACGGCGGAGCCAAGCGCATCGAGAGACGGCTATTAAGCCGAT
GATACATGTCAGTGCACAAAGAAGAACCGCAAGGATGAGGACGAGGTCGGAGTTGAAAGG
CGGAGAAGCTTCAGCCGGAGATGTAGAATTGGCTTCTTGGAGAA

Last Updated on 06.12.2018 16:11