Sequences from confirmation amplicon


Confirmation sequences for line SALK_032917c and hit At3g02780 are shown.
Line ID SALK_032917c
Locus At3g02780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:602443
Primers used for PCR 33fr - LBb3, show primer details
   
Sequence Name 53-K111957-SALK_032917C-33fr
GenBank Accession LN485582 [GenBank]
Sequencing primer gene specific primer, 33fr: 5'-GTGGGTATCTACTGGCAAAACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K111957-SALK_032917C-33fr
AACTGTAGCTTTTCTCAACTAGTGACCTCACTGTTACTCATCAGGTGTGAGGAATGCTGC
ACAAAGAAAGCTTCTCGATGAGCTTGGTATTGTAGCTGAAGATGTACCAGTCGATGAGTT
CACTCCCTTGGGACGTATGCTGTACAAGGCTCCTTCTGATGGCAAATGGGGAGAGCATGA
ACGTAAATACCATTTCTCAAGACTCTTGGTTCCCATTTTCTTGAACAGAATAATAAAACC
TTAAGCTTCTTCTGTGTTGGCCTTCCTTTTGCAGTTGATTACTTGCTCTTCATCGTGCGA
GACGTGAAGGTTCAACCAAACCCAGATGAAGTAGCTGAGATCAAGTATGTGAGCCGGGAA
GAGCTGAAGGAGCTGGTGAAGAAAGCAGATGCAGGTGAGGAAGGTTTGAAACTGTCACCA
TGGTTCAGATTGGTGGTGGACAATTTCTTGATGAAGTGGTGGGATCATGTTGAGAAAGGA
ACTTTGGTTGAAGCTATAGACATGAAAACCATCCACAAACTCTGAACATCTTTTTTTAAA
GTTTTTAAATCAATCAACTTTCTCTTCATCATTTTTATCTTTTCGATGATAATAATTTGG
GATATGTGAGACACTTACAAAACTTCCAAGCACCTCAGGCAATAATAAAGTTTTCGTTTT
TAAACAAATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGG
GTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCC
TGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTG
ATGGTGG
   
Sequence Name 49-K111956-SALK_032917C-R210
GenBank Accession LN485480 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K111956-SALK_032917C-R210
CATTTGTTAAAACGAAACTTTATTATTGCCTGAGGTGCTTGGAAGTTTTGTAAGTGTCTC
ACATATCCCAAATTATTATCATCGAAAAGATAAAAATGATGAAGAGAAAGTTGATTGATT
TAAAAACTTTAAAAAAAGATGTTCAGAGTTTGTGGATGGTTTTCATGTCTATAGCTTCAA
CCAAAGTTCCTTTCTCAACATGATCCCACCACTTCATCAAGAAATTGTCCACCACCAATC
TGAACCATGGTGACAGTTTCAAACCTTCCTCACCTGCATCTGCTTTCTTCACCAGCTCCT
TCAGCTCTTCCCGGCTCACATACTTGATCTCAGCTACTTCATCTGGGTTTGGTTGAACCT
TCACGTCTCGCACGATGAAGAGCAAGTAATCAACTGCAAAAGGAAGGCCAACACAGAAGA
AGCTTAAGGTTTTATTATTCTGTTCAAGAAAATGGGAACCAAGAGTCTTGAGAAATGGTA
TTTACGTTCATGCTCTCCCCATTTGCCATCAGAAGGAGCCTTGTACAGCATACGTCCCAA
GGGAGTGAACTCATCGACTGGTACATCTTCAGCTACAATACCAAGCTCATCGAGAAGCTT
TCTTTGTGCAGCATTCCTCACACCTGATGAGTAACAGTGAGGTCACTAGTTGAGAAAAGC
TACAGTTTTCTACATAGATNGTAAAAAGAAGTACTAAATTGTTTTGCC

Last Updated on 06.12.2018 16:11