Sequences from confirmation amplicon


Confirmation sequences for line SALK_033579c and hit At4g05450 are shown.
Line ID SALK_033579c
Locus At4g05450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2759242
Primers used for PCR 52qz - LBb3, show primer details
   
Sequence Name 64-K116104-SALK_033579C-52qz
GenBank Accession LN485855 [GenBank]
Sequencing primer gene specific primer, 52qz: 5'-TTATAGACCACCCAATTTCTCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K116104-SALK_033579C-52qz
AACCAAATTAGGACTCTTTCCAACTGGACACATTAGAATCGTCACTAACAGATCCATCTA
ACACCAAACAAGCGCAGAAAAGCTACATTTCGTTCTTCTGCGATCACTTTATGACATTAC
TAACGAGATTCTACCTAGCCAGATAATAGAGTGAACTCTAACAAAGAATCCTATTCTAAG
TATTAATGAGAGTTTATACATGAAGAAGTAACAAAATTATCGATTGTAAGAGAGTATAAA
ACTCGAGAACAAGCTCAGACTAACCAAAGACTGCAAGTAGTGGCCATAAGACCGGTGTAG
ATTCTTTGTCCCGTATGTTGCTAAATATCCCTCTGCCAAAACAAGAAATAGAAAGAGCAG
CAAAATATTAAGGCAGCATTGTGTAGGCAGACAATGTGGACAAATATATTGTGGTGTAAA
CAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGG
TTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAG
AGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGG
TGGTTCCG
   
Sequence Name 59-K116103-SALK_033579C-R210
GenBank Accession LN485723 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K116103-SALK_033579C-R210
TTTGTTTCCCACATATATTTGTCCTCATTGTCTGCCTACACAATGCTGCCTTATAAAGTT
GCTGCTCTTTCTATTTCTTGTTTTGGCAGAGTGGATATTTAGCAACATACGGGACATAGA
ATCTACACCGGTCTTATGGCCACTACTTGCAGTCTTTGGTTAGTCTGAGCTTGTTCTCGA
GTTTTATACTCTCTTACAATCGATAATTTTGTTACTTCTTCATGTATAAACTCTCATTAA
TACTTAGAATAGGATTCTTTGTTAGAGTTCACTCTATTATCTGGCTAGGTAGAATCTCGT
TAGTAATGTCATAAAGTGATCGCAGAAGAACGAAATGTAGCTTTTCTGCGCTTGTTTGGT
GTTAGATGGATCTGTTAGTGACGATTCTAATGTGTCCAGTTGGAAAGAGTCCTATATTTG
GTTTTGGCTCAAAGGCCTAAAAAAAGGCTTAAGGTGAGAAATTGGGTGGTCTATAA

Last Updated on 06.12.2018 16:11