Sequences from confirmation amplicon


Confirmation sequences for line SALK_034924c and hit At3g15540 are shown.
Line ID SALK_034924c
Locus At3g15540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5264526
Primers used for PCR 64tv - LBb3, show primer details
   
Sequence Name 28-K117180-SALK_034924C-64tv
GenBank Accession LN485093 [GenBank]
Sequencing primer gene specific primer, 64tv: 5'-CAACGAAGCAACATATAAAAAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K117180-SALK_034924C-64tv
CTCATAGAGAGTGTAGGAGAAGAAGTTCTCATTTCATAATTGTATCAAATTGTGAGAGGA
AAAAAAGAAGTTCAAGAAATGGAGAAGGAAGGACTCGGGCTTGAGATAACGGAGCTGAGA
TTGGGGCTTCCGGGGAGAGATGTGGCAGAGAAGATGATGAAGAAGAGAGCTTTCACGGAG
ATGAATATGACGTCGTCGGGTAGTAATAGTGATCAATGTGAAAGCGGCGTCGTTTCATCT
GGTGGTGACGCTGAGAAGGTTAATGATTCGCCGGCGGCGAAAAGCCAGGTGGTGGGGTGG
CCACCGGTTTGTTCTTACCGGAAGAAAAACAGCTGTAAGGAAGCTTCGACCACGAAAGTG
GGGTTAGGGTATGTGAAAGTGAGCATGGATGGTGTGCCTTATTTGAGGAAGATGGATCTT
GGTTCGAGCCAAGGCTATGATGATCTAGCCTTTGCTCTTGATAAGCTCTTCGGTTTCCGT
GGCATCGGTTAGTAACAATTATGTTGCGAACAGGATATATTGTGGTGTAAACAAATTGAC
GCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTT
TCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCA
GCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 07-K117179-SALK_034924C-R210
GenBank Accession LN484471 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K117179-SALK_034924C-R210
ACCACAATATATCCTGTTCGCAACATAATTGTTACTAACCGATGCCACGGAAACCGAAGA
GCTTATCAAGAGCAAAGGCTAGATCATCATAGCCTTGGCTCGAACCAAGATCCATCTTCC
TCAAATAAGGCACACCATCCATGCTCACTTTCACATACCCTAACCCCACTTTCGTGGTCG
AAGCTTCCTTACAGCTGTTTTTCTTCCGGTAAGAACAAACCGGTGGCCACCCCACCACCT
GGCTTTTCGCCGCCGGCGAATCATTAACCTTCTCAGCGTCACCACCAGATGAAACGACGC
CGCTTTCACATTGATCACTATTACTACCCGACGACGTCATATTCATCTCCGTGAAAGCTC
TCTTCTTCATCATCTTCTCTGCCACATCTCTCCCCGGAAGCCCCAATCTCAGCTCCGTTA
TCTCAAGCCCGAGTCCTTCCTTCTCCATTTCTTGAACTTCTTTTTTTCCTCTCACAATTT
GATACAATTATGAAATGAGAACTTTCTTCTCCTACACTTCTCTTATTGTAGTAATGGCAC
CGCGTCGTGCTTTTTATAT

Last Updated on 06.12.2018 16:11