Sequences from confirmation amplicon


Confirmation sequences for line SALK_038994c and hit At1g08230 are shown.
Line ID SALK_038994c
Locus At1g08230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2585968
Primers used for PCR 55us - LBb3, show primer details
   
Sequence Name 08-K117668-SALK_038994C-R210
GenBank Accession LN484503 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K117668-SALK_038994C-R210
CATTATTTGTTTCATTGGTATGAGCTCAACTTTTGTTCGTGGTTGGTTAATATCACAATA
TATGTATTGTGTAAACCTATGATTTTTAAAAAAATACTTTTCTATACTGTTTGGTCTTGT
TTCGAATTATAAGAAGAAAAAAAACTAATCATATGGAATTCATATTATAGTGAATTTTTT
GATATGGCTTGCTTCGGTTCAATTTTGTTGGGATCCGGATCGAAGTGTTGACCATTCCAC
ATTTCCACTCATACCACTGTTAAGTTTCAACTTTTTCATGCGTTAGATAGCTGTCTCTTT
CCGTGTCTTGTTTCATTTTTGATGCAGAAATTTGTCTTTCAAAACTAATTTCAATTTTTA
AAAATATGTGCGTGTCAGAAGAGTATTCATAAAACGTTGGTGCCGACCAAAATTTAATCT
TATATAAGTAAAGTTGGATAGGTTCGATCTGCTTTCAAAAGTAGAATATTCTAAATTTCA
GATCCTACATAGGATTCGATC
   
Sequence Name 45-K117669-SALK_038994C-55us
GenBank Accession LN485399 [GenBank]
Sequencing primer gene specific primer, 55us: 5'-TCCTAATTTGGATCGAATCCTATG-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K117669-SALK_038994C-55us
TTTTGAAGCGATCGAACCTATCCAACTTTACTTATATAAGATTAAATTTTGGTCGGCACC
AACGTTTTATGAATACTCTTCTGACACGCACATATTTTTAAAAATTGAAATTAGTTTTGA
AAGACAAATTTCTGCATCAAAAATGAAACAAGACACGGAAAGAGACAGCTATCTAACGCA
TGAAAAAGTTGAAACTTAACAGTGGTATGAGTGGAAATGTGGAATGGTCAACACTTCGAT
CCGGATCCCAACAAAATTGAACCGAAGCAAGCCATATCAAAAAATTCACTATAATATGAA
TTCCATATGATTAGTTTTTTTTCTTCTTATAATTCGAAACAAGACCAAACAGTATAGAAA
AGTATTTTTTTAAAAATCATAGGTTTACACAATACATATATTGTGATATTAACCAACCAC
GAACAAAAGTTGAGCTCATACCAATGTAAACAAATTAATTGACGCTTAGACAACTTAATA
ACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGC
AACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTG
GTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA

Last Updated on 06.12.2018 16:11