Sequences from confirmation amplicon


Confirmation sequences for line SALK_042239 and hit At1g11990 are shown.
Line ID SALK_042239
Locus At1g11990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4048512
Primers used for PCR 17hd - LBb3, show primer details
   
Sequence Name 67-K112564-SALK_042239C-17hd
GenBank Accession LN485930 [GenBank]
Sequencing primer gene specific primer, 17hd: 5'-TTGGTAGCTCCTTTACTATCCGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K112564-SALK_042239C-17hd
TTCTCTTGGTAGATATCTCCAACTGACTATATATGAAGTATTAATTAAAGTTCAATACAT
CGTTACAAATGTAAAAACCTCAAAAATGGCTAGAAAATGAGAGATAGAGGACGGAGAGCT
TACAATAGTTACCTTGCATCTGTCCAAACGTCACTTAACATGAATTTGGGAATGACGAGA
GCCGCATTGAGCAATCGAGCTACAACAACAATGTTACATACCTATGACGAAATCCATTGG
TTATAGACTTATAGTCACTAGAGAATTTGAAAGATAAATTTGAAGGACAAGGAGAAGAAA
ACTTACAGCAACTCTTTGCTGATTAATCCCTCCGTTTGCAGTTACCATAATATATCCATT
TTTGCCATCTAAAAAATCAGGCAAGTTAAACTCCATAGGAAACAAGCTTTAACCTAATTT
TATAAAGAGAGAGATTGAATGAGACCGTCAGGAGTCAAAGAACGTTGATCCGCACAAGGC
TTCCACGCCGAAGCTTGTTCCTTCGGCTCCTGCCATAAGTCTTTGGTCTCAAGTTTGTTC
TGCTCAAAATCAAAACCATTACAATAACGGTTATCACGCATTCCCTATTAAAAAGAACAA
AACAATGTTACTGAGAATTAGGAAGGATAAGATTGGTTTGTGAAGAATATATTTACCTCT
GCCAAAGCATCAGTAGCCAAACTCAACATTCTACCATATGTTCCATTATATTTCATCTTC
TGTTTTTCAAACTTTTTACGCTCTTCATTCTGATTCACACAACANAAAGTTTAGATCANA
TGACGCTNAAACAAATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATG
TACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGC
CTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATC
CTGTTTGATGGTGGTTCC
   
Sequence Name 51-K112563-SALK_042239C-R210
GenBank Accession LN485537 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K112563-SALK_042239C-R210
TTTGTTTAGCGTCATTTGATCTAACTTTTTGTTGTGTGAATCAGAATGAAGAGCGTAAAA
AGTTTGAAAAACAGAAGATGAAATATAATGGAACATATGGTAGAATGTTGAGTTTGGCTA
CTGATGCTTTGGCAGAGGTAAATATATTCTTCACAAACCAATCTTATCCTTCCTAATTCT
CAGTAACATTGTTTTGTTCTTTTTAATAGGGAATGCGTGATAACCGTTATTGTAATGGTT
TTGATTTTGAGCAGAACAAACTTGAGACCAAAGACTTATGGCAGGAGCCGAAGGAACAAG
CTTCGGCGTGGAAGCCTTGTGCGGATCAACGTTCTTTGACTCCTGACGGTCTCATTCAAT
CTCTCTCTTTATAAAATTAGGTTAAAGCTTGTTTCCTATGGAGTTTAACTTGCCTGATTT
TTTAGATGGCAAAAATGGATATATTATGGTAACTGCAAACGGAGGGATTAATCAGCAAAG
AGTTGCTGTAAGTTTTCTTCTCCTTGTCCTTCAAATTTATCTTTCAAATTCTCTAGTGAC
TATAAGTCTATAACCAATGGATTTCGTCATAGGTATGTAACATTGTTGTTGTAGCTCGAT
TGCTCAATGCGGCTCTCGTCATTCCCAAATTCATGTTAAGTGACGTTTGGACAGATGCAA
GGTAACTATTGTAAGCTCTCCGTCCTCTATCTCTCATTTTCTAGCCATTTTTGAGGTTTT
TACATTTGTAACGATGTATTGAACTTTAATTAATACTTCATATATAGTCAGTTTGGAGAT
ATCTACCAAGAGGAACATTTTATGGAGTACTTGTCGCCCGATATTCGGATAGTAA

Last Updated on 06.12.2018 16:11