Sequences from confirmation amplicon


Confirmation sequences for line SALK_045957c and hit At1g77000 are shown.
Line ID SALK_045957c
Locus At1g77000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28940654
Primers used for PCR 35dr - LBb3, show primer details
   
Sequence Name 92-K111079-SALK_045957C-35dr
GenBank Accession LN486347 [GenBank]
Sequencing primer gene specific primer, 35dr: 5'-AACAGAGACATACCAAGAGAGGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K111079-SALK_045957C-35dr
CAGGAACGCATCTCTCCGCCACTACAATACAAGAAGCAATGATCACAGTCCGATCATCAA
CAAGGTTTAAAATCTTCATGAGAAGCTCCACAGGGATATCTTTCCACTCAGAGATCAAAA
CTCCTTCCATCTTCATATTCTCGAAACAGAGGTTAAGTTCTTTTCTTGTTGCTCCTTCAC
TCACCATCCTTGAAGCGGTTTCTTTGATTCTAACACCACGAGATCAAAAGTAAATATGAG
AATGAGAAATGAGAGAGAAAGAAGATAAATAAATATCTCTGAGTCTATGACATCAAAAGA
CGACAAATGTAAGGAAATAAGGATTGAGTGAGATATGGTCTTAAAACTCTTTAAACTAAA
CTGCAAGCATTAATGTCTCTCTATGTGTAACTCTTTTTTAGGACATTAATAAACAAAACC
CCATAATTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTT
CTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGT
TGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
CCGA
   
Sequence Name 90-K111078-SALK_045957C-R210
GenBank Accession LN486334 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K111078-SALK_045957C-R210
GGGTTTTGTTATAATGTCCTAAAAAGAGTTACACATAGAGAGACATTAATGCTTGCAGTT
TAGTTTAAAGAGTTTTAAGACCATATCTCACTCAATCCTTATTTCCTTACATTTGTCGTC
TTTTGATGTCATAGACTCAGAGATATTTATTTATCTTCTTTCTCTCTCATTTCTCATTCT
CATATTTACTTTTGATCTCGTGGTGTTAGAATCAAAGAAACCGCTTCAAGGATGGTGAGT
GAAGGAGCAACAAGAAAAGAACTTAACCTCTGTTTCGAGAATATGAAGATGGAAGGAGTT
TTGATCTCTGAGTGGAAAGATATCCCTGTGGAGCTTCTCATGAAGATTTTAAACCTTGTT
GATGATCGGACTGTGATCATTGCTTCTTGTATTTGTAGTGGCTGGAGAGATGCTGTTTCC
CTTGGCCTCACTCGCCTCTCCCTCTCTTGGTAGATCTCTGT

Last Updated on 06.12.2018 16:11