Sequences from confirmation amplicon


Confirmation sequences for line SALK_047438c and hit At1g59900 are shown.
Line ID SALK_047438c
Locus At1g59900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22051331
Primers used for PCR 55cs - LBb3, show primer details
   
Sequence Name 86-K109033-SALK_047438C-55cs
GenBank Accession LN486294 [GenBank]
Sequencing primer gene specific primer, 55cs: 5'-AGCAATACCACAACCTAATGGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K109033-SALK_047438C-55cs
AAAACGACGATTCCTTCTTATAGAAGTGCATAGATCCACCTTTCCCTTTACACTACCAGC
NTTGTCTTCCCATAAGCTCTGAGAAAACCTCATGAAGCGAACCACCACGACCTAAGAAAA
TACAGTGATCACGATAAGCAGTAATAATCGCGTCTTTCTTTGTAATCGCAGCTTCCATGC
CTATAGCTACAGCTTCTTGGCCGTCGTAGAGATGACAAAACCCTCGGATTAGCTTCGCTT
TGTAAAGCGAATCGGCTGCGATTTCCATACGTCGCATCAACGCCATGGTTCTGAAGAAAT
CGAGAAGCTCTTGACTCGATGATTCGACGGAGCGTGACGGTGGATCACATAAGTGAGCTG
TGAAAGGAAGCGAAGTCTCGATTGTGATCGGAGTTGTGTCCGTTGAGATTAATCGGCTGA
AGGCGGCGGAGAAGGGACGAGTGATGATGTTGGATCGTGATGAGAGGCGTGATAGAGCCA
TTTCTCCGCCGTTGATTCGAAGATTCTCCGGCGAATCTTAATAACACATTGACGCTTAGA
CAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAG
TGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCG
GTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGATTGATGGTGGTTCCG
   
Sequence Name 70-K109032-SALK_047438C-R210
GenBank Accession LN485999 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K109032-SALK_047438C-R210
AGATTCGCCGGAGAATCTTCGAATCAACGGCGGAGAAATGGCTCTATCACGCCTCTCATC
ACGATCCAACATCATCACTCGTCCCTTCTCCGCCGCCTTCAGCCGATTAATCTCAACGGA
CACAACTCCGATCACAATCGAGACTTCGCTTCCTTTCACAGCTCACTTATGTGATCCACC
GTCACGCTCCGTCGAATCATCGAGTCAAGAGCTTCTCGATTTCTTCAGAACCATGGCGTT
GATGCGACGTATGGAAATCGCAGCCGATTCGCTTTACAAAGCGAAGCTAATCCGAGGGTT
TTGTCATCTCTACGACGGCCAAGAAGCTGTAGCTATAGGCATGGAAGCTGCGATTACAAA
GAAAGACGCGATTATTACTGCTTATCGTGATCACTGTATTTTCTTAGGTCGTGGTGGTTC
GCTTCATGAGGTTTTCTCAGAGCTTATGGGAAGACAAGCTGGTTGTTCTAAAGGGAAAGG
TGGATCTATGCACTTCTATAAGAAGGAATCGTCGTTTTACGGTGGTCATGGGATTGTTGG
TGCTCAGGTTCCATTAGGTTGTGGTATTGC

Last Updated on 06.12.2018 16:11