Sequences from confirmation amplicon


Confirmation sequences for line SALK_048263c and hit At1g55205 are shown.
Line ID SALK_048263c
Locus At1g55205 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20595447
Primers used for PCR 28ux - LBb3, show primer details
   
Sequence Name 21-K117668-SALK_048263C-R210
GenBank Accession LN484895 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K117668-SALK_048263C-R210
CATTTGTTTACCCACAATATATCCTGAAAACTCTACTTGTGATTGAAATTGTTGTCTTTG
TTGGCTTATAGTTTACTTGTATTTGCAGTTTTGGTTATGTTTAATACTTTGGCATATGCA
TCATGTTTAGTTCTTGGGCATTGGTTTCATCTTGTGGAGTCTTCTTATATTTGATATGAA
CTTGTTGCTTATCTTTATGCATTTAAAAGTTAACCTTTTCTATCTGAAACCGCAGGAATT
TCAATGCCTTTATGACGGTATTTCATCTTACAACAAGCTTTGGGTATGATAAAGACATGA
AATGAAAACAAAAACTCGGGTTTGTATCGTTCTCGTTATCTCTTATCAAACCCAAAGGGT
CCTGAATTTAGAGAACAGGTCCTAAAACTCTCCGCTTAAATTCTTTCATTTTCGAG
   
Sequence Name 58-K117669-SALK_048263C-28ux
GenBank Accession LN485698 [GenBank]
Sequencing primer gene specific primer, 28ux: 5'-CTCGAAAATGAAAGAATTTAAGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K117669-SALK_048263C-28ux
GACCCTTTGGGTTTGATAAGAGATAACGAGAACGATACAAACCCGAGTTTTTGTTTTCAT
TTCATGTCTTTATCATACCCAAAGCTTGTTGTAAGATGAAATACCGTCATAAAGGCATTG
AAATTCCTGCGGTTTCAGATAGAAAAGGTTAACTTTTAAATGCATAAAGATAAGCAACAA
GTTCATATCAAATATAAGAAGACTCCACAAGATGAAACCAATGCCCAAGAACTAAACATG
ATGCATATGCCAAAGTATTAAACATAACCAAAACTGCAAATACAAGTAAACTATAAGCCA
ACAAAGACAACAATTTCAATCACAAGTAGAGTTTTCAGGATATATTGTGGTGTAAACAAA
TTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTT
TCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAG
TTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGT
TCCGAATGGGGCAAAAAT

Last Updated on 06.12.2018 16:11