Sequences from confirmation amplicon


Confirmation sequences for line SALK_049267 and hit At1g79180 are shown.
Line ID SALK_049267
Locus At1g79180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29787632
Primers used for PCR 10en - LBb3, show primer details
   
Sequence Name 27-K111273-SALK_049267C-10en
GenBank Accession LN485059 [GenBank]
Sequencing primer gene specific primer, 10en: 5'-TTCAGAGGAGGAAGAAACAATCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K111273-SALK_049267C-10en
CAGCTAGGATTATTATTGTGCTCATACATATATGGTGGATCAAAAAAGTGATTTGATATT
GACATTAAGCTTTCTGCATTTTGATCAGGTGGTCGAAAATCGCTTCTCAACTTCCAGGTA
GAACAGATAACGAGATCAAGAATGTGTGGCACACTCATCTAAAGAAAAGACTGGCTCAGA
GCTCAGGAACTGCAGATGAACCGGCCTCGCCTTGTTCGAGTGATTCTGTTTCTCGTGGGA
AAGATGATAAGTCATCTCACGTAGAAGATTCTTTGAACAGAGAGACTAATCATAGGAATG
AGTTGTCTACATCTATGTCTTCTGGGGGTTCCAACCAACAAGATGATCCAAAGATAGACG
AACTCAGGTTTGAGTATATAGAAGAAGCTTATAGCGAGTTTAACGACATTATTATTCAAG
AGGTAGACAAACCCGATCTGCTGGAGATACCATTTGATTCAGATCCTGACATTTGGAGTT
TCTTAGATACTTCAAACTCATTTCAACAATCCACTGCAAATGAGAACAGCTCAGGCTCAA
GAGCAACAACAGAAGAAGAGTCTGATGAGGATGAGGTTAAGAAATGGTTCAAGCACCTAG
AAAGCGAACTCGGGTTAGAAGAAGACGATNATCAACAACAATACAAAGAAGAAGAATCAT
CATCATCATCACTCTTGAAGAACTACGAGCTCATGATACATTGATAAAAGATATTTATTT
TGGATATCAATATGACAATATGTATGACAATATTCCAGGATATATTGTGGTGTAAACAAA
TTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTT
TCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAG
TTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGT
T
   
Sequence Name 11-K111272-SALK_049267C-R210
GenBank Accession LN484580 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K111272-SALK_049267C-R210
CATTTGTTTACCCCAATATATCCTGGAATATTGTCATACATATTGTCATATTGATATCCA
AAATAAATATCTTTTATCAATGTATCATGAGCTCGTAGTTCTTCAAGAGTGATGATGATG
ATGATTCTTCTTCTTTGTATTGTTGTTGATTATCGTCTTCTTCTAACCCGAGTTCGCTTT
CTAGGTGCTTGAACCATTTCTTAACCTCATCCTCATCAGACTCTTCTTCTGTTGTTGCTC
TTGAGCCTGAGCTGTTCTCATTTGCAGTGGATTGTTGAAATGAGTTTGAAGTATCTAAGA
AACTCCAAATGTCAGGATCTGAATCAAATGGTATCTCCAGCAGATCGGGTTTGTCTACCT
CTTGAATAATAATGTCGTTAAACTCGCTATAAGCTTCTTCTATATACTCAAACCTGAGTT
CGTCTATCTTTGGATCATCTTGTTGGTTGGAACCCCCAGAAGACATAGATGTAGACAACT
CATTCCTATGATTAGTCTCTCTGTTCAAAGAATCTTCTACGTGAGATGACTTATCATCTT
TCCCACGAGAAACAGAATCACTCGAACAAGGCGAGGCCGGTTCATCTGCAGTTCCTGAGC
TCTGAGCCAGTCTTTTCTTTAGATGAGTGTGCCACACATTCTTGATCTCGTTATCTGTTC
TACCTGGAAGTTGAGAAGCGATTTTCGACCACCTGATCAAAATGCAGAAAGCTTAATGTC
AATATCAAATCACTTTTTTGATCCACCATATATGTATGAGCACAATAATAATCCTAGCTG
ATGATTTACTTGTTCCCATAGTTGTGGTGAAAAGCTTATGATGTTTCT

Last Updated on 06.12.2018 16:11