Sequences from confirmation amplicon


Confirmation sequences for line SALK_055283c and hit At4g25970 are shown.
Line ID SALK_055283c
Locus At4g25970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13185241
Primers used for PCR 03tw - LBb3, show primer details
   
Sequence Name 15-K116922-SALK_055283C-03tw
GenBank Accession LN484704 [GenBank]
Sequencing primer gene specific primer, 03tw: 5'-AGATGATTTTCCTTTTCTTGATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K116922-SALK_055283C-03tw
TGAGCTTGATCTACTTGATTTCGTAGTACAGGTCAGTAACTAAAGTTTGTTTTTTCATAT
GAAAGCTGTTTAAGTTGTTTATCCGTTATTCCTCTAGCTCGACTAATAAGTATCAGCTAG
TTTTTTCTATCACTTATCTTCCATTGTTTGAGGAGATTCAGCCACAGTTTAGCTGATAAT
AGTAGGTGCATGGATTAGATATCTTGAATGATTAATCGACGAGAAACTTGTTTGTTTACA
TGTTTATTTTATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGC
GGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA
TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCC
AGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 04-K116921-SALK_055283C-R210
GenBank Accession LN484380 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K116921-SALK_055283C-R210
CCACAATATAAAATAAACATGTAAACAAACAAGTTTCTCGTCGATTAATCATTCAAGATA
TCTAATCCATGCACCTACTATTATCAGCTAAACTGTGGCTGAATCTCCTCAAACAATGGA
AGATAAGTGATAGAAAAAACTAGCTGATACTTATTAGTCGAGCTAGAGGAATAACGGATA
AACAACTTAAACAGCTTTCATATGAAAAAACAAACTTTAGTTACTGACCTGTACTACGAA
ATCAAGTAGATCAAGCTCACAATAACCAACGATATTGTTCTTCAAGAGTCTATTGGTCTG
CATCAAGAAAAGAAAAATCATC

Last Updated on 06.12.2018 16:11