Sequences from confirmation amplicon


Confirmation sequences for line SALK_060558c and hit At4g29240 are shown.
Line ID SALK_060558c
Locus At4g29240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14418327
Primers used for PCR 36dr - LBb3, show primer details
   
Sequence Name 65-K110715-SALK_060558C-36dr
GenBank Accession LN485869 [GenBank]
Sequencing primer gene specific primer, 36dr: 5'-TTGCTGCCTTTCTTCTTCTTAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K110715-SALK_060558C-36dr
TTTCAGAGTTTGACCTTAAGGAAAGAGAATGAATAGTGATTGTTTCAGTGGATTAATGAG
TTTTGTGTGAGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGAGAGAGTGTGAGATTAT
GAAACACAGAGTGGATGTGGTTGAAGACGCATTGATGATATGAGAGAGGGCACAAGATTC
TCAAAAAGAGCTCTTATTTGCCCCTTGTGTAGTTATCTCACATTTGCCTTTGTATTGTTA
AGTTTCACTGTTTATGCTCAACTTATGATTGCATGAATCATATCACGGGTTTCTCCTCTT
TTTCCTTTTGCCCCACTTTGTTATTTCAACGCATTTTAGTGTAAAATATTGACTCACATG
TTTTCACAAATTGTCACATTTCTCATTTGAATGGACGCACTACTTTGACCCCCCTCTTGA
TAAGTTCACTAATAATGGTTTTTAAGCCCCACCCACCAAACAAGCTCTAATTGATTGTGG
TGTAAACAAATTCACGCTTACACCACTTAATCACACATTGCGCACGTTTTTAATGCACTG
GGGTGGTTTCTCTTCTCACCAGAGAGACGGGCAACAGCTGATTGCCCCTCACCGCCTGGC
CCTGAGAGAGTCGCAGCACGCGGCCCACTCTGGTCTGCCCCCGCACGCG
   
Sequence Name 49-K110714-SALK_060558C-R210
GenBank Accession LN485477 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K110714-SALK_060558C-R210
GTCATTTGTTTACCCCAATCAATTAGAACTTGTTTAGTAGGTGGAACTTAAAAACCATTA
TTAGTTAACTTATCAAGAACGGAATCAAACTAGTGCATCCATTCAAATGACAAATGTAAC
AATTTGTCAAAACATGTGATTCAATATTTTACACTAAAATGCATTGAAATAACAAAGTGG
AACAAAAGGAAAAAGAAGAAAAACCCGTGATATGATTCATGCAATCATAAGTTGAACATA
AACAGTAAAACTTAACAATACAAAGGCAAATGTGACATAACTACACAAGGAACAAATAAG
AACTCTTTTTGATAATCTTGTCCCCTCTCTCATATCATCAATGCTTCTTCAACCACATCC
ACTCTGTTTTTCATAATCTCACACTCTCTCTCTCTCTCTCTCTCTCTCTCACACACACAC
ACTCACACAAAACTCATTAATCCACTGAAACAATCACTATTCACTCTCTTTCCTTAAGTG
TCAAACTCTTGAAAAAACTACTTTGAAGGAAAAAATGTCTAAAAAGAAGAAAGGCAGCA

Last Updated on 06.12.2018 16:11