Sequences from confirmation amplicon


Confirmation sequences for line SALK_063593c and hit At5g64640 are shown.
Line ID SALK_063593c
Locus At5g64640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25838220
Primers used for PCR 13ii - LBb3, show primer details
   
Sequence Name 29-K112623-SALK_063593C-13ii
GenBank Accession LN485107 [GenBank]
Sequencing primer gene specific primer, 13ii: 5'-GTAAAAACGGAGGGAAAGATTGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K112623-SALK_063593C-13ii
TTCCGCGCCGGATACAACAGAACTGTAAAGTTTGTGATACGTATTAGAGAAGGTGTGTAT
GAAGAAACCGTTAGGGTTCCGTTTGAGAAAAAGAATGTCGTCTTCATCGGTGACGGAATG
GGTAAAACGGTAATTACAGGGTCGTTGAATGTTGGACAACCAGGGATGACAACGTTTGAA
TCTGCAACTGTCGGTGAGTTTTTTTCAACCTCGTTAAACCTTTCTTTAATTAAGTTCTTA
TTTTTCTCGTCAGTTTATTAATTTAATTAAGGAACATAAATAAGAAGAATAACATTAAAG
TCAAAACATAAATTTGTCTTGTTTCTTTTACTGTGTCTAGATTTGCTCTGCCACGTAAAA
AGAATAATATTTATTGTTATTTAGTTTTGAGATTCAAGTTATGTATGTCTATAGAATTAT
TATGAGACTGTCCCTGGTTCCACTAGGGACAGAAACCATGTGACATTTACTTGGGACAAA
GAATAACACATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTG
GGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGC
CCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTT
TGATGGTGGTTCCGA
   
Sequence Name 05-K112622-SALK_063593C-R210
GenBank Accession LN484400 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K112622-SALK_063593C-R210
ATTCTTTGTCCCAAGTAAATGTCACATGGGTTTCTGTCCCTAGTGGAACCAGGGACAGTC
TCATAATAATTCTATAGACATACATAACTTGAATCTCAAAACTAAATAACAATAAATATT
ATTCTTTTTACGTGGCAGAGCAAATCTAGACACAGTAAAAGAAACAAGACAAATTTATGT
TTTGACTTTAATGTTATTCTTCTTATTTATGTTCCTTAATTAAATTAATAAACTGACGAG
AAAAATAAGAACTTAATTAAAGAAAGGTTTAACGAGGTTGAAAAAAACTCACCGACAGTT
GCAGATTCAAACGTTGTCATCCCTGGTTGTCCAACATTCAACGACCCTGTAATTACCGTT
TTACCCATTCCGTCACCGATGAAGACGACATTCTTTTTCTCAAACGGAACCCTAACGGTT
TCTTCATACACACCTTCTCTAATACGTATCACAAACTTTACAGTTCTGTTTGTATCCGGC
GCGGAATCAACGGCTTCTTGCACTGTTTTGTATTTACAATCTTTCCCTCCGTTTTTA
Primers used for PCR 33gz - LBb3, show primer details
   
Sequence Name 85-K112710-SALK_063593C-33gz
GenBank Accession LN486286 [GenBank]
Sequencing primer gene specific primer, 33gz: 5'-CATGATGTTATCGTTCGACAGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K112710-SALK_063593C-33gz
CGACGAAGAGACGGGTTTTGGGAGAAGCTGGGCCGAGTTTGGGCTCGGGTACGGGTACAG
AGGCTAGTTTGGGTTTTCCGTCTGGTTTAACGGAAGACGTGACGGTGTGTAAAAACGGAG
GGAAAGATTGTAAATACAAAACAGTGCAAGAAGCCGTTGATTCCGCGCCGGATACAAACA
GAACTGTAAAGTTTGTGATACGTATTAGAGAAGGTGTGTATGAAGAAACCGTTAGGGTTC
CGTTTGAGAAAAAGAATGTCGTCTTCATCGGTGACGGAATGGGTAAAACGGTAATTACAG
GGTCGTTGAATGTTGGACAACCAGGGATGACAACGTTTGAATCTGCAACTGTCGGTGAGT
TTTTTTCAACCTCGTTAAACCTTTCTTTAATTAAGTTCTTATTTTTCTCGTCAGTTTATT
AATTTAATTAAGGAACATAAATAAGAAGAATAACATTAAAGTCAAAACATAAATTTGTCT
TGTTTCTTTTACTGTGTCTAGATTTGCTCTGCCACGTAAAAAGAATAATATTTATTGTTA
TTTAGTTTTGAGATTCAAGTTATGTATGTCTATAGAATTATTATGAGACTGTCCCTGGTT
CCACTAGGGACAGAAACCATGTGACATTTACTTGGGACAAAGAATAACACATTGACGCTN
AGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCAC
CAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAA
GCGGTCCACGCTGGTNTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTG
   
Sequence Name 75-K112709-SALK_063593C-R210
GenBank Accession LN486130 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K112709-SALK_063593C-R210
ATTCTTTGTCCAAGTAAATGTCACATGGGTTTCTGTCCCTAGTGGAACCAGGGACAGTCT
CATAATAATTCTATAGACATACATAACTTGAATCTCAAAACTAAATAACAATAAATATTA
TTCTTTTTACGTGGCAGAGCAAATCTAGACACAGTAAAAGAAACAAGACAAATTTATGTT
TTGACTTTAATGTTATTCTTCTTATTTATGTTCCTTAATTAAATTAATAAACTGACGAGA
AAAATAAGAACTTAATTAAAGAAAGGTTTAACGAGGTTGAAAAAAACTCACCGACAGTTG
CAGATTCAAACGTTGTCATCCCTGGTTGTCCAACATTCAACGACCCTGTAATTACCGTTT
TACCCATTCCGTCACCGATGAAGACGACATTCTTTTTCTCAAACGGAACCCTAACGGTTT
CTTCATACACACCTTCTCTAATACGTATCACAAACTTTACAGTTCTGTTTGTATCCGGCG
CGGAATCAACGGCTTCTTGCACTGTTTTGTATTTACAATCTTTCCCTCCGTTTTTACACA
CCGTCACGTCTTCCGTTAAACCAGACGGAAAACCCAAACTAGCCTCTGTACCCGTACCCG
AGCCCAAACTCGGCCCAGCTTTCTCCCAAAACCCGTCTCTTTCCGTCGCTGGACGAATCC
ACGAAACGACGTCGTCTCCGAAACTGTCGAACGATAACATCATG

Last Updated on 06.12.2018 16:11