Sequences from confirmation amplicon


Confirmation sequences for line SALK_064185 and hit At3g42730 are shown.
Line ID SALK_064185
Locus At3g42730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:14845799
Primers used for PCR CF96 - R204, show primer details
   
Sequence Name 59-K107135-SALK_064185C-CF96
GenBank Accession LN485708 [GenBank]
Sequencing primer gene specific primer, CF96: 5'-AATTAGAAAGCGAAACGAAATTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K107135-SALK_064185C-CF96
CTTCGATTGTTGGGTAGTTGAATTGAGTCACTGAACTCCCACATCCGGGGAAGAGAAAGT
TACTGTTTTTCCCGGCGAAGCCAAGAAAAAGTTACTGTTTATTCCGACGAAATTTGAACC
TCCGGCGAGATAGCAACTTTAGCTATGGCTGGGAATAAAGCAAACGAAGAAGTCCCGAGG
AAGCAGCATGGTCCAGGGAAGGAGAAGCGGGTATTAAGAGTTTCCCCGCAGAGAAAACGC
TCTGCGACAAAGGCAACGGAGGAACTGGGTTAGAATCTCTAATTTACCGTTTGTTTTCTT
TGGGTGTATCAGAATTGTAAAGCTTGTTTGATTAAAGGGTTAGAACTAGTCGGAATTTAG
CATATCTTTAGGATGAAAAATTGAGAAGTAGAATTGGAAATCTCTGTTTCCGCCAAAAAT
ATGATTTTGAGTTATAATCTGGAAAATGTCTGTGTACGTACACTACTTCGGTTTGCTTAA
CAATATATCACGTTTAATTGCAGGTAGTCCAAGTGTTGCTCCGATTGAAGATCTTACAGT
TGAAGAGTTACCACCAAGACTCTTTGCTATAGACCGGTACCCTTCCGAGACAAAGATGAA
CGCGTACTCTAAACCGGAGTACATTTCTAACATAGCAAATGTACTAAAGGGGAAACCAGA
GATGCAGTTTCTTCTCGATTCTCCATTTGGTGTAAACAAATTGACGCTTAGACAACTTAA
TAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGG
GCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCACAGGG
   
Sequence Name 51-K107134-SALK_064185C-R210
GenBank Accession LN485525 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K107134-SALK_064185C-R210
GTCATTTGTTTCACCAAATGGAGAATCGAGAAGAACTGCATCTCTGGTTTCCCCTTTAGT
ACATTTGCTATGTTAGAAATGTACTCCGGTTTAGAGTACGCGTTCATCTTTGTCTCGGAA
GGGTACCGGTCTATAGCAAAGAGTCTTGGTGGTAACTCTTCAACTGTAAGATCTTCAATC
GGAGCAACACTTGGACTACCTGCAATTAAACGTGATATATTGTTAAGCAAACCGAAGTAG
TGTACGTACACAGACATTTTCCAGATTATAACTCAAAATCATATTTTTGGCGGAAACAGA
GATTTCCAATTCTACTTCTCAATTTTTCATCCTAAAGATATGCTAAATTCCGACTAGTTC
TAACCCTTTAATCAAACAAGCTTTACAATTCTGATACACCCAAAGAAAACAAACGGTAAA
TTAGAGATTCTAACCCAGTTCCTCCGTTGCCTTTGTCGCAGAGCGTTTTCTCTGCGGGGA
AACTCTTAATACCCGCTTCTCCTTCCCTGGACCATGCTGCTTCCTCGGGACTTCTTCGTT
TGCTTTATTCCCAGCCATAGCTAAAGTTGCTATCTCGCCGGAGGTTCAAATTTCGTCGGA
ATAAACAGTAACTTTTTCTTGGCTTCGCCGGGAAAAACAGTAACTTTCTCTTCCCCGGAT
GTGGGAGTTCAGTGACTCAATTCAACTACCCAACAATTCGAAGTTATGTCGAATTTTGAA
ACAATGGATACTGAATTTCG
Primers used for PCR 75xo - LBb3, show primer details
   
Sequence Name 75-K119302-SALK_064185C-75xo
GenBank Accession LN486143 [GenBank]
Sequencing primer gene specific primer, 75xo: 5'-TGGTTTTCTTCTGGGTGAAATAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K119302-SALK_064185C-75xo
TTATACTTACCTATCTCTTCTTGTTGTGGTGGTCGCACNCTGCTTTGGTACTCTAGCATA
GCCTCCAACCCATTCATCCTCCTGCCACTGGTGCCCATCTATCAACAGTGCCTCAATGTA
ATCAACCCTTGGATCATCAACTTCATCATCCCATGAAAGAGATGGAGGACAAACACTATC
AGCTGGTTTCACAATATAGTTGACCTCAACCTACACAAATAAGTATGTTAACCATAGATA
ATGTACATTTACTTATTATGTACGTACATACACAATTTAACACACCTCATCAGCAGCCTC
ACACTCCAAAAAACGAGATGTCCGGATTGCCCGCAGTGAGGCAAGACGATGGATAGACCT
CTCAGCAAATGTCCTGTTAGGTACATCATCAGGTCCTAGCTTAGCAATTGATGGGATTGT
TTCGAAAGCTAAAAGATGGAGAGCTAATGGGAAGCCATGTAGTGCATAAGACCCTACCAG
TAAACCCCTTATTAGTTGTTGAGCATCGTAAGGAGTTTGGAATTTTGCTATCCTCCCCAA
AGTACGGGTAAAGGACACCCTTCCCCATGGGTATTTGCAGAAGAACTCCAGGTTCTTCGT
CATCTCGACAGTCTTCGAAGTAGCCCTGTTGGGGTTACTGTGGGCCGCTACCACACCGTC
TACCAAAATAATAAGAGATAGTGCCAGTTGCTTCCACCCCTCCATGCTTTCCTCCTCCTG
CAGCCAGCTCACCAAATCAGCAATTGTTGGAACCGTGTCCCCAAACCTCTCATCGAACAG
AGTCTTCCAAACACTCTCACACTCAGGTTTCACACTGATAACATCCTCCACGTCTTTCTT
CTTGGGATACTTCCCACATTCCAGCCCAGTTAGGATACTAAATTCTCTTAATCCAAATCT
GATTGGATGACCACCGAATACAATCCACAT
   
Sequence Name 27-K119301-SALK_064185C-R210
GenBank Accession LN485076 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K119301-SALK_064185C-R210
GTCATTTGTTTCACCAAAAACAAGGCATCTTTCATGCTAAGCTTGTCCTTGGACTAATAT
GCTGTCAGTTAGTGACAAAGAAGGTGAATGAGATGTGGATTGTATTCGGTGGTCATCCAA
TCAGATTTGGATTAAGAGAATTTAGTATCCTAACTGGGCTGGAATGTGGGAAGTATCCCA
AGAAGAAAGACGTGGAGGATGTTATCAGTGTGAAACCTGAGTGTGAGAGTGTTTGGAAGA
CTCTGTTCGATGAGAGGTTTGGGGACACGGTTCCAACAATTGCTGATTTGGTGAGCTGGC
TGCAGGAGGAGGAAAGCATGGAGGGGTGGAAGCAACTGGCACTATCTCTTATTATTTTGG
TAGACGGTGTGGTAGCGGCCCACAGTAACCCCAACAGGGCTACTTCGAAGACTGTCGAGA
TGACGAAGAACCTGGAGTTCTTCTGCAAATACCCATGGGGAAGGGTGTCCTTTACCCGTA
CTTTGGGGAGGATAGCAAAATTCCAAACTCCTTACGATGCTCAACAACTAATAAGGGGTT
TACTGGTAGGGTCTTATGCACTACATGGCTTCCCATTAGCTCTCCATCTTTTAGCTTTCG
AAACAATCCCATCAATTGCTAAGCTAGGACCTGATGATGTACCTAACAGGACATTTGCTG
AGAGGTCTATCCATCGTCTTGCCTCACTGCGGGCAATCCGGACATCTCGTTTTTTGGAGT
GTGAGGCTGCTGATGAGGTGTGTTAAATTGTGTATGT

Last Updated on 06.12.2018 16:11