Sequences from confirmation amplicon


Confirmation sequences for line SALK_068498c and hit At4g25990 are shown.
Line ID SALK_068498c
Locus At4g25990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13192386
Primers used for PCR 31jr - LBb3, show primer details
   
Sequence Name 76-K113914-SALK_068498C-31jr
GenBank Accession LN486153 [GenBank]
Sequencing primer gene specific primer, 31jr: 5'-CAAAGAGCTCTGAAGTTACGGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K113914-SALK_068498C-31jr
CCGTTCCTTCCGACGAGATTCTTGGTTCGGACGCTGACGGAGTCGATTTTCATGTACGTT
ACTTCAATTTCTCTATTTTCTCATTTATATTAGCCTTATCTTAATAATTTGAATCGAAAT
AAAAACGTTTTGATGACCAATTCGTTGGCAACAAACAATTCATTATGAATTTCTGAACCT
GACAAGAATTGAAAATGGTTCTTATGTAGTTCTTGTCATTTCTGACTTACAATGTGTTAT
TGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATG
TACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGC
CTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATC
CTGTTTGATGGTGGTTCC
   
Sequence Name 65-K113913-SALK_068498C-R210
GenBank Accession LN485875 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K113913-SALK_068498C-R210
CCACAAAACACATCGTAAGTCAGAAAAGAACAAGAACTACATAAGAACCATTTTTCAATT
CTTGTCAGGTTCCAGAAATTCATAATGAATTCGTTCGTTCGCCAACAATCGGTCATCAAA
ACGTTTTTATTTCGATTCAAATTATTAAGATAAGGCTAATATAAATGAGAAAATAGAGAA
TTGAAGTAACGTACATGAAAATCGACTCCGTCAGCGTCCGAACCAAGAATCTCGTCGGAG
AACGGTGACTCCTTACCTGACCAAGCCTCTAAAACCCCATCGTAGTCGAGCTTCAGCAAC
GGACTCACTCTCTGCTCCAGCTTTGGATTCGAGTCCGTAACTTCGAAACCTCTTTG

Last Updated on 06.12.2018 16:11