Sequences from confirmation amplicon


Confirmation sequences for line SALK_068598 and hit At1g20460 are shown.
Line ID SALK_068598
Locus At1g20460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7092808
Primers used for PCR 24td - LBb3, show primer details
   
Sequence Name 73-K116811-SALK_068598C-24td
GenBank Accession LN486088 [GenBank]
Sequencing primer gene specific primer, 24td: 5'-CACTCCATTTTTAAGTTCAGGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K116811-SALK_068598C-24td
ATACAGTCCCACAGTGTTATGTAGTTAAGCCGATAGTACGTCTCGTTTTTCAAGATTCAG
TCCCATTATATGATGCTAAATTGGTCGAAATTCTCTTCCTTCCATCTCTTACAATCATGA
TTGAGTCCTTTCTTGTTTGACTTCTACTTATCAAGCTTCACTAACATCTAGACATCTTCA
TGAGTTATACATTGTTAACAAGCTTCTATATCGTTTGATCCAGGGTGGATGTTGTAGTCT
TCGTCAACAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACAT
TGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGC
TGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGC
CCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCCAGCAAAATA
   
Sequence Name 73-K116810-SALK_068598C-R210
GenBank Accession LN486087 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K116810-SALK_068598C-R210
TATATCCTGTTGACGAAGACTACAACATCCACCCTGGATCAAACGATATAGAAGCTTGTT
AACAATGTATAACTCATGAAGATGTCTAGATGTTAGTGAAGCTTGATAAGTAGAAGTCAA
ACAAGAAAGGACTCAATCATGATTGTAAGAGATGGAAGGAAGAGAATTTCGACCAATTTA
GCATCATATAATGGGACTGAATCTTGAAAAACGAGACGTACTATCGGCTTAACTACATAA
CACTGTGGGACTTGTATACTAGGATAGGCAAGTACGTGACATATATTATATTTTACAATA
ATTAGCTCCTGAACTTAAAAATGGAGTG

Last Updated on 06.12.2018 16:11