Sequences from confirmation amplicon


Confirmation sequences for line SALK_077395c and hit At5g59770 are shown.
Line ID SALK_077395c
Locus At5g59770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24079903
Primers used for PCR CF27 - R212, show primer details
   
Sequence Name 49-K105819-SALK_077395C-CF27
GenBank Accession LN485468 [GenBank]
Sequencing primer gene specific primer, CF27: 5'-TTCTTTGTTTGAAAAGCTGGAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K105819-SALK_077395C-CF27
AGGACGGCCTGAATAAAATGACATGGAAAGATCAGCATGGTTGACCTTTTATGAAACAGA
ATCCAAAAATGCTGCTAAAAACATAAAAGCTATATATCCATAAACGATACTTCAGCCAAA
AGGATTTTGTTGAAGGTGAAGAAGAAGAAGGAAGCGATGAACATACCCAAAGGAAATCGT
AGTAACTGAAGGGGAAAACAGAGAAGAAATTAGCGTAAAGATTCCTTTCTTTAACATATG
GAAGTGCTTTGTACATGAGCAGCTGGAAACGACAAACACTGATAGTTTAAACTGAAGGCG
GGAAACGACAATCTGATCATGAGCGGAGAATTAAGGGAGTCACGTTATGACCCCCGCCGA
TGACGCGGGACAAGCCGTTTTACGTTTGGAACTGACAGAACCGCAACGTTGAAGGAGCCA
CTCAGCCGCGGGTTTCTGGAGTTTAATGAGCTAAGCACATACGTCAGAAACCATTATTGC
GCGTTCAAAATCGNCCTAAGG
   
Sequence Name 65-K105820-SALK_077395C-R214
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K105820-SALK_077395C-R214
TACGATTCTCAAGCTCACGGAGTAGGTCTCGCTCCGGATGTTTCCCGCCCTCTCTGCATA
CAAACACGATGGATCTTT
Primers used for PCR CF26 - R204, show primer details
   
Sequence Name 50-K105819-SALK_077395C-CF26
GenBank Accession LN485492 [GenBank]
Sequencing primer gene specific primer, CF26: 5'-AGTGTTTTCACCAGTGTGATTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K105819-SALK_077395C-CF26
TTTTGGTCAGGATTCGCCGTGGCTGTCTATAACACTTGTGGCTTGGTGTATCGGTGAGGT
AAGTTTAATCTTTACAATAATAAAACAGAGAAATGCCAATGACAAGTTTGTGACTACTGA
ATTGAAACATGTTAGTTATCTCAAGAGAACTTTTTTATAGCAGAATAGATTACTGTCTAG
GTGGTTGATGATGTTATATACAACTAGCTGTATACCACCAAATGTGTTAGCTGAGTGATG
TATTTGGATGTAGATGATCAGATATCCTCATTATGCTTTTACTTGCCTCGGTAGATGTCC
CTATTGGCTAACCTACCTCAGGTATTGCATCATACTTTGTATATTGTTTTGAACTGAAAC
ATTCCCTATCACAGAAAGTATTTTCGAATTGAATGGATTGCGTGATATGTTTGACCATCC
AGGTACACGGGATTCATCGTGATTTATCCAACGGGACTAGTGGGTGAATGTGAGTGCTTT
AAAGCTAGAATTCTTGTTTTCATTTCTTCTTGTTGATATTGACCATTGTGGTGTAAACAA
ATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTT
TTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGA
GTTGCAGCAACGGGTCCACG
   
Sequence Name 66-K105820-SALK_077395C-R210
GenBank Accession LN485887 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K105820-SALK_077395C-R210
GTCATTTGTTTACCACAATGGTCAATATCAACAAGAAGAAATGAAAACAAGAATTCTAGC
TTTAAAGCACTCACATTCACCCACTAGTCCCGTTGGATAAATCACGATGAATCCCGTGTA
CCTGGATGGTCAAACATATCACGCAATCCATTCAATTCGAAAATACTTTCTGTGATAGGG
AATGTTTCAGTTCAAAACAATATACAAAGTATGATGCAATACCTGAGGTAGGTTAGCCAA
TAGGGACATCTACCGAGGCAAGTAAAAGCATAATGAGGATATCTGATCATCTACATCCAA
ATACATCACTCAGCTAACACATTTGGTGGTATACAGCTAGTTGTATATAACATCATCAAC
CACCTAGACAGTAATCTATTCTGCTATAAAAAAGTTCTCTTGAGATAACTAACATGTTTC
AATTCAGTAGTCACAAACTTGTCATTGGCATTTCTCTGTTTTATTATTGTAAAGATTAAA
CTTACCTCACCGATACACCAAGCCACAAGTGTTATAGACAGCCACGGCGAATCCTGGACC
TAAAAAGATGAATGTAAAAACAGAATCACACTGGTGAAAAACAC

Last Updated on 06.12.2018 16:11