Sequences from confirmation amplicon


Confirmation sequences for line SALK_086345c and hit At3g49320 are shown.
Line ID SALK_086345c
Locus At3g49320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18290622
Primers used for PCR 13lu - LBb3, show primer details
   
Sequence Name 61-K114145-SALK_086345C-R210
GenBank Accession LN485770 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K114145-SALK_086345C-R210
CACCAAACATGGCTAAGAGAGAGAAGAAGAAGCTCTTCTACACTAAACCCTCGACGTCGC
GGGTTTAAGCTTGACATTATAACGCCGTCGTTTCGTCAACTTTGGGATTTGATAAAACGA
CGCCGTATGTGTTTTCTTTGTGAAAGACTAAAACGACGCCGTTTGAATTTTACATTTTTG
GAATTATTTTCTTAGGGAATCTTCTAATAGAGTTGATGATCTTTGGTTGACCTACCAAAT
ATGGTTGATCACCACACTGACCACAGTTTGGTTATCAATTATCAAAATTCTGTTTATATC
ATATTAAAGTGATTTCATGTACCAAATGTATTGTTTTAATCGTGGGACCTTAAGTTATAA
TCATAGAAAACTCAGGATCAATTCCAAATG
   
Sequence Name 72-K114146-SALK_086345C-13lu
GenBank Accession LN486061 [GenBank]
Sequencing primer gene specific primer, 13lu: 5'-CATTTGGATTTGATCCTGAGTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K114146-SALK_086345C-13lu
ACATACATTTGGTACATGAAATCACTTTAATATGATATAAACAGAATTTTGATAATTGAT
AACCAAACTGTGGTCAGTGTGGTGATCAACCATATTTGGTAGGTCAACCAAAGATCATCA
ACTCTATTAGAAGATTCCCTAAGAAAATAATTCCAAAAATGTAAAATTCAAACGGCGTCG
TTTTAGTCTTTCACAAAGAAAACACATACGGCGTCGTTTTATCAAATCCCAAAGTTGACG
AAACGACGGCGTTATAATGTCAAGCTTAAACCCGCGACGTCGAGGGTTTAGTTGTAGAAG
AGCTTCTTCTTCTCTCTCTTAGCCATGTTTGGTGTAAACAAATTGACGCTTAGACAACTT
AATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGAC
GGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCAC
GCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC

Last Updated on 06.12.2018 16:11