Sequences from confirmation amplicon


Confirmation sequences for line SALK_087882c and hit At5g17810 are shown.
Line ID SALK_087882c
Locus At5g17810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5880894
Primers used for PCR 83sm - LBb3, show primer details
   
Sequence Name 46-K116586-SALK_087882C-83sm
GenBank Accession LN485420 [GenBank]
Sequencing primer gene specific primer, 83sm: 5'-CTGCAGAAATTAACCTCAAGAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K116586-SALK_087882C-83sm
GTTTTTCGTAGAAAATAATATCCGTAACCGAAGTTTTCGTCGATTTAACTAAGCTTTACG
GATTCGATGCAGCTCATATATATGGATCGTATATATGTGACCTTTTTTATTTGGTCCAAG
TTTTTGATAAATATAAATATGTAGTATATATTATTTATTTTTAAAAATAAAGAATATAGC
CAAAAACACAAGAACCAATAACTCCACTTTTGAGTAATTTTGTAAAATATTGAGTTCATT
GGTCGAAGAGACCTTGCGGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGG
GTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCC
TGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTG
ATGGT
   
Sequence Name 29-K116585-SALK_087882C-R210
GenBank Accession LN485113 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K116585-SALK_087882C-R210
TTTTTTGGCTATATTCTTTATTTTTAAAAATAAATAATATATACTACATATTTATATTTA
TCAAAACTTGGACCAAATAAAAAAGGTCACATATATACATCCATATATATGAGCTGCATC
AATCCGTAAAGCTTAGTTAAATCACAAAACTTCAGTTACAGATATTAATTTCTACAAAAA
CGATATTCATTTATTACATGCATAAACCGACAAACATCAAATTGATTTATATTTTATTAT
ATTTTCTGCAGAAATATTTTTATGTTATAGTTCCAGAGAGTGAGATGGGTTCTTGA

Last Updated on 06.12.2018 16:11