Sequences from confirmation amplicon


Confirmation sequences for line SALK_089262c and hit At3g53780 are shown.
Line ID SALK_089262c
Locus At3g53780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19925879
Primers used for PCR 14gq - LBb3, show primer details
   
Sequence Name 75-K112564-SALK_089262C-14gq
GenBank Accession LN486128 [GenBank]
Sequencing primer gene specific primer, 14gq: 5'-GTTCTCGGTTACAGCTCCATTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K112564-SALK_089262C-14gq
ACATGTTCGTTTCCATCGACATTGTTGAAGAGAGAGATCAATCCAACAATGAACCTGATA
AGGAAAGAAGCTTGGTTTGGTTTTCTCACAAAGTTCATAACTTTTTTAGTGTCAATCAAA
GAGACAAGATCATCATTTACACAATCTGCAGGCTTTTATTAGTATAGTTAAAAACAAACA
GACTGATTTATTGCTATTGACGTGACAGAGAAATGTAACACTTACCCAGCGACCAAGATG
AGAAGAGAGATAGTCCATAATATGCCCTGATATATCTTGAACCTGTGGGGTTTAGCTCCA
GGACCGTTTCTTTGGTTGATCCATCCGTAATGGGGACGGATTAAGAGCACAAATCCAAGA
AGAAAACCTGTAGCAAACCCTCCGATATGAGCAAAGTTATCTACTCCAGGGAGCACACCG
AGCCCCAAGTTCACCGCCACAATCAATACAAGAGTTACAATCGTCACAACCTAGAAAAAG
CATTGGCAAAACAACATAAATTCTAAAGTTCAGATCTTTAGATCATAGATAGAAAACAGG
TCTTGAAGGTATAGTGAGTTACCTTGTTAGAATAGATTGTCCAGTTGATAAAGATCTCAG
AGAGCATTCCTCCAAGTAAACCAAACACGGCACCAGATGCTCCAACAGAGATGTTTGAAC
GGAGGAAAAGAGCTGACAATATGCTTCCTCCAAACCCTGATATCAAATAAAGCAATCCAA
TCCGAACTGCAAACAAAAATCAAAACAAAGGTTATATACTTACACACACACTTCACTAAA
AAAGGATCTTCACAAGCTAACAAAGTGATCAAGAGAGGCAGAGAGAGTAGTTAACTCACT
AAAACCAAATTCACGTTCCATACGGAACACATTGCGGACGTTTTTAATGTACTGGGGTGG
TTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAG
AGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTG
   
Sequence Name 59-K112563-SALK_089262C-R210
GenBank Accession LN485715 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K112563-SALK_089262C-R210
TGGTTTTGTGAGTTACTACTCTCTCTGCCTCTCTTGATCACTTTGTTAGCTTGTGAAGAT
CCTTTTTTAGTGAAGTGTGTGTGTAAGTATATAACCTTTGTTTTGATTTTTGTTTGCAGT
TCGGATTGGATTGCTTTATTTGATATCAGGGTTTGGAGGAAGCATATTGTCAGCTCTTTT
CCTCCGTTCAAACATCTCTGTTGGAGCATCTGGTGCCGTGTTTGGTTTACTTGGAGGAAT
GCTCTCTGAGATCTTTATCAACTGGACAATCTATTCTAACAAGGTAACTCACTATACCTT
CAAGACCTGTTTTCTATCTATGATCTAAAGATCTGAACTTTAGAATTTATGTTGTTTTGC
CAATGCTTTTTCTAGGTTGTGACGATTGTAACTCTTGTATTGATTGTGGCGGTGAACTTG
GGGCTCGGTGTGCTCCCTGGAGTAGATAACTTTGCTCATATCGGAGGGTTTGCTACAGGT
TTTCTTCTTGGATTTGTGCTCTTAATCCGTCCCCATTACGGATGGATCAACCAAAGAAAC
GGTCCTGGAGCTAAACCCCACAGGTTCAAGATATATCAGGGCATATTATGGACTATCTCT
CTTCTCATCTTGGTCGCTGGGTAAGTGTTACATTTCTCTGTCACGTCAATAGCAATAAAT
CAGTCTGTTTGTTTTTAACTATACTAATAAAAGCCTGCAGATTGTGTAAATGATGATCTT
GTCTCTTTGATTGACACTAAAAAAGTTATGAACTTTGTGAGAAAACCAAACCAAGCTTCT
TTCCTTATCAGGTTCATTGTTGGATTGATCTCTCTCTTCAACAATGTCGATGGAAACGAA
CATTGTTCGTGGTGTCATTATCTTTCATGTGTTCCAACATCTAAATGGAGCTGTAACC

Last Updated on 06.12.2018 16:11