Sequences from confirmation amplicon


Confirmation sequences for line SALK_093745c and hit At5g56050 are shown.
Line ID SALK_093745c
Locus At5g56050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22701169
Primers used for PCR 26ty - LBb3, show primer details
   
Sequence Name 39-K117180-SALK_093745C-26ty
GenBank Accession LN485284 [GenBank]
Sequencing primer gene specific primer, 26ty: 5'-TTGGGATTGCTACTTTGATTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K117180-SALK_093745C-26ty
CTCTATGCAAAGCTCAACACCATCCTCTTTGAGTCACCGGTTTATTTCAACGGTGATATG
TTGTTGCAGTTAAATTTCACCAATCCTAACAAGAAACTAAATGTGAGGTTTGAGAATCTT
ATGGTAGAGCTCTGGTTTGCCGACACAAAGATCGCCACACAAGGTGTCTTGCCGTTTTCG
CAAAGAAACGGCAAGACTAGGCTAGAACCGATCCGTTTGATTTCAAATTTGGTCTTCTTG
CCTGTAAATCATATACTTGAGCTCAGAAGACAAGTGACGAGCAATAGGATAGCTTATGAA
ATCAGAAGTAACTTCAGAGTCAAAGCTATTTTTGGGATGATTCATTATTCTTATATGCTA
CACGGGATTTGTCAGCTTCAATTGAGCAGTCCACCAGCCGGTGGCTTAGTTTATCGGAAC
TGCACTACAAAAAGATGGTGATAAACAAATTGACGCTTAGACAACTTAATAACACATTGC
GGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA
TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCC
AGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCCGGCAAAAAT
   
Sequence Name 18-K117179-SALK_093745C-R210
GenBank Accession LN484792 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K117179-SALK_093745C-R210
ACCATCTTTTTGTAGTGCAGTTCCGATAAACTAAGCCACCGGCTGGTGGACTGCTCAATT
GAAGCTGACAAATCCCGTGTAGCATATAAGAATAATGAATCATCCCAAAAATAGCTTTGA
CTCTGAAGTTACTTCTGATTTCATAAGCTATCCTATTGCTCGTCACTTGTCTTCTGAGCT
CAAGTATATGATTTACAGGCAAGAAGACCAAATTTGAAATCAAACGGATCGGTTCTAGCC
TAGTCTTGCCGTTTCTTTGCGAAAACGGCAAGACACCTTGTGTGGCGATCTTTGTGTCGG
CAAACCAGAGCTCTACCATAAGATTCTCAAACCTCACATTTAGTTTCTTGTTAGGATTGG
TGAAATTTAACTGCAACAACATATCACCGTTGAAATAAACCGGTGACTCAAAGAGGATGG
TGTTGAGCTTTGCATTAGAGATGTCAAAAACAGGGGTTCTTGGTTTGACGGCGAGATAGA
GAATCAAAGTACCAATCCCAA

Last Updated on 06.12.2018 16:11