Sequences from confirmation amplicon


Confirmation sequences for line SALK_099974 and hit At4g02200 are shown.
Line ID SALK_099974
Locus At4g02200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:974167
Primers used for PCR 44wj - LBb3, show primer details
   
Sequence Name 23-K118627-SALK_099974C-44wj
GenBank Accession LN484960 [GenBank]
Sequencing primer gene specific primer, 44wj: 5'-ATCGATGAGCCGTTGTCTACTAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K118627-SALK_099974C-44wj
TATTATCATTTATTACATCCGCCGTCACCAAAGAAGTCCAAGCTTGTACAACCTGATTCA
TCTAGTGAAGCAAGCATGGAAGACAATAGCTTAATAAGGGATTCAACAGAAAAGTAAGTA
ATTCTTTGACATTTTCTTGATTCTGCTTGCTTTATCTCATCTTCTTAAACCTTGTCTAAA
GATACATTTCGTATTTCTGTGTGCTCAGAGACTGGGAATCGCCGTCTCCCTTGTCAGATA
CAGAGCTACTAGAGAAGGCAAAGAAGAGAGAGTTTGTACAAGGTTTAATATCACATTGAC
GCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTT
TCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCA
GCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA
ATCGTGCAAAA
   
Sequence Name 12-K118626-SALK_099974C-R210
GenBank Accession LN484627 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K118626-SALK_099974C-R210
TATTAACCTTGTACAACTCTCTCTTCTTTGCCTTCTCTAGTAGCTCTGTATCTGACAAGG
GAGACGGCGATTCCCAGTCTCTGAGCACACAGAAATACGAAATGTATCTTTAGACAAGGT
TTAAGAAGATGAGATAAAGCAAGCAGAATCAAGAAAATGTCAAAGAATTACTTACTTTTC
TGTTGAATCCCTTATTAAGCTATTGTCTTCCATGCTTGCTTCACTAGATGAATCAGGTTG
TACAAGCTTGGACTTCTTTGGTGACGGCGGATTGTATATAAATGATAATAATGGGTCCAG
AACACTTTTAGATGTATGATTAGTAGACAACGGNTCATCGATA

Last Updated on 06.12.2018 16:11