Sequences from confirmation amplicon


Confirmation sequences for line SALK_101062c and hit At2g23130 are shown.
Line ID SALK_101062c
Locus At2g23130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9844852
Primers used for PCR 04gq - LBb3, show primer details
   
Sequence Name 18-K113191-SALK_101062C-04gq
GenBank Accession LN484782 [GenBank]
Sequencing primer gene specific primer, 04gq: 5'-AGAACAAGTAGAGACCATGCGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K113191-SALK_101062C-04gq
CACACTTATTCTTTGTGCTCCGCTCTGCANAAAATAATTCGAAATTTTAAGTTCTAGAAA
CTAGAAAGAATCAAATCTAACAAAAAAATTGTGATTTCAGACATTTTTAAATTATAACGA
ATAACCTAAACTATAGTCAAAAGATGGGACTAATTTACATTTGTTTTTACTTAAGTGATA
TTTTTACATATTACCTAAATTAATTTACCATATTGTTTAGGTTAAATCATATATTATTTA
CCTGATCGTCAGCGGCAGGAGAGAAAGCATCGCTTGGTACAAATTGTGGACAAATTGACG
CTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTT
CACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAG
CAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAA
TGGGGCAAAAA
   
Sequence Name 07-K113190-SALK_101062C-R210
GenBank Accession LN484462 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K113190-SALK_101062C-R210
TACCAGCGATGCTTTCTCTCCTGCCGCTGACGATCCGCTTTTAATATATGATTTAACCTA
AACAATATGGTAAATTAATTTCTTTAATATGTAAAAATATCACTTAAGTAAAAACAAATG
TAAATTAGTCCCATCTTTTGACTATAGTTTAGGTTATTCGTTATAATTTAAAAATGTCTG
AAATCACAATTTTTTTGTTAGATTTGATTCTTTCTAGTTTCTAGAACTTAAAATTTCGAA
TTATTTTACTGCAGAGCGGAGCACAAAGAATAAGTGTTGTGATACAAATGGTGGGAGCTG
CGGCAATCGCATGGTCTCTACTTGTTCT

Last Updated on 06.12.2018 16:11