Sequences from confirmation amplicon


Confirmation sequences for line SALK_110091 and hit At4g33010 are shown.
Line ID SALK_110091
Locus At4g33010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15927185
Primers used for PCR CE50 - R212, show primer details
   
Sequence Name 57-K107135-SALK_110091C-CE50
GenBank Accession LN485656 [GenBank]
Sequencing primer gene specific primer, CE50: 5'-GAAGCATTTTTCTTTTCATCTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K107135-SALK_110091C-CE50
TTCTACCAAAGTTTGTGTTTTGTTTTCGGTCAATTGTGGAATATATAATTTATAGAAGAT
GTATTCGAATGAAATAATAGAGTAAGAGACAGAAAGCGTATAATGAGAACACTATGGACA
GAATGTGAAAGAGAAGAGTGCGTGGTGAGAGAAAGGGACACAACACAATGGGACAGAGCA
GTAATCAAGCAGACACTGCAGCTGCGACTTGTTCTTCCTCTGGGAGGAGAGTGCACACCA
GTTTCCTGTCTCCATATACATTGTCCACACGCCCTGTTCCACATTTCAATCATTTGTCAC
ATATCAGAATTCCGAAACCAGAATAAACTGAATCGAAAACCAAATGTGCAATCTATATCT
TGGATGGTCTAATACCTGTGGTGGGCCAGAACTTGGAGGAGCGGAGCCAAGGCGCAGGGA
AAGCAGCATACTCTCGAGAATACGGCTTTTTCCATGTGTCTGCCATTAGCAACGATGGGG
GATGTGGAGCTCCCTGAATCTCAAGGGAGAATCTGATAGTTTAAACTGAAGGCGGGAAAC
GACAATCTGATCATGAGCGGAGAATTAAGGGAGTCACGTTATGACCCCCGCCGATGACGC
GGGACAAGCCGTTTTACGTTTGGAACTGACAGAACCGCAACGTTGAAGGAGCCACTCAGC
CGCGGGTTTCTGGAGTTTAATGAGCTAAGCACATACGTCAGAAACCATTATTGCGCGTT
   
Sequence Name 49-K107134-SALK_110091C-R214
GenBank Accession LN485471 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K107134-SALK_110091C-R214
GCGGGGGTCATAACGTGACTCCCTTAATTCTCCGCTCATGATCAGATTGTCGTTTCCCGC
CTTCAGTTTAAACTATCAGATTCTCCCTTGAGATTCAGGGAGCTCCACATCCCCCATCGT
TGCTAATGGCAGACACATGGAAAAAGCCGTATTCTCGAGAGTATGCTGCTTTCCCTGCGC
CTTGGCTCCGCTCCTCCAAGTTCTGGCCCACCACAGGTATTAGACCATCCAAGATATAGA
TTGCACATTTGGTTTTCGATTCAGTTTATTCTGGTTTCGGAATTCTGATATGTGACAAAT
GATTGAAATGTGGAACAGGGCGTGTGGACAATGTATATGGAGACAGGAAACTGGTGTGCA
CTCTCCTCCCAGAGGAAGAACAAGTCGCAGCTGCAGTGTCTGCTTGATTACTGCTCTGTC
CCATTGTGTTGTGTCCCTTTCTCTCACCACGCACTCTTCTCTTTCACATTCTGTCCATAG
TGTTCTCATTATACGCTTTCTGTCTCTTACTCTATTATTTCATTCGAATACATCTTCTAT
AAATTATATATTCCACAATTGACCGAAAACAAAACACAAACTTTGGTAGAATCAAGACTT
AAATCAACTATACTGCCATAGACGCAAGATGAAAAGAAAA
Primers used for PCR CE49 - R204, show primer details
   
Sequence Name 58-K107135-SALK_110091C-CE49
GenBank Accession LN485681 [GenBank]
Sequencing primer gene specific primer, CE49: 5'-TGAGAGGCTTCAAGGTATGAAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K107135-SALK_110091C-CE49
GTTTGGGTATGCATATCACTGACCGATTCAAATATGAATTTGTAGAACACTGCTGGAATA
GAACCAGAGGATGTGGCGAAACGGCTAATGGACTATGGATTCCATGGACCCACAATGTCT
TGGCCTGTCCCTGGAACTCTTATGATTGAGCCAACCGAGAGTGAAAGCAAGGTAAATATG
GATATATGCAGATCTCTCATTGACTCTTTCTTCTCTCAGTGTATCAACTTGATGAAACTA
AATAATATTCGTTTGCAGGCGGAGCTAGACAGGTTCTGCGATGCTCTCATTTCAATCAGG
GAAGAAATTGCACAGATTGAAAAAGGAAATGCAGATGTCCAGAACAACGTTCTCAAGGTA
ATTAGGAAAACAAAGACTTATCATAATTATATCCAATAATAATATTGTTCGGAGAAGTTG
AGATTCATAACAAATTGTGGTGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAAT
GTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCG
CCTGGCCCTGAGAGAGTTGCAG
   
Sequence Name 50-K107134-SALK_110091C-R210
GenBank Accession LN485496 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K107134-SALK_110091C-R210
ACATTTGTATGAATCTCAACTTCTCCGAACAATATTATTATTGGATATAATTATGATAAG
TCTTTGTTTTCCTAATTACCTTGAGAACGTTGTTCTGGACATCTGCATTTCCTTTTTCAA
TCTGTGCAATTTCTTCCCTGATTGAAATGAGAGCATCGCAGAACCTGTCTAGCTCCGCCT
GCAAACGAATATTATTTAGTTTCATCAAGTTGATACACTGAGAGAAGAAAGAGTCAATGA
GAGATCTGCATATATCCATATTTACCTTGCTTTCACTCTCGGTTGGCTCAATCATAAGAG
TTCCAGGGACAGGCCAAGACATTGTGGGTCCATGGAATCCATAGTCCATTAGCCGTTTCG
CCACATCCTCTGGTTCTATTCCAGCAGTGTTCTACAAATTCATATTTGAATCGGTCAGTG
ATATGCATACCCAAAACAACTACTTTAATTTCAGTTCACAACATTTTCATACCTTGAGGN
CCTCTC

Last Updated on 06.12.2018 16:11