Sequences from confirmation amplicon


Confirmation sequences for line SALK_118908c and hit At5g49760 are shown.
Line ID SALK_118908c
Locus At5g49760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20220994
Primers used for PCR 59vi - LBb3, show primer details
   
Sequence Name 60-K117945-SALK_118908C-59vi
GenBank Accession LN485751 [GenBank]
Sequencing primer gene specific primer, 59vi: 5'-CTCAGATGAGACGACAACCATAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K117945-SALK_118908C-59vi
TGGGATCGTTGATGTGTTAGGTTGCAAACCCAATAACTTTATTACTTTTTTTTCTTATTA
GTTAATACTAATTTTACTTACGTACTACAAATTATTTGTAATATAAATTTGTAATATAAT
CTCATGCATAAATGTGAAAGGTTTGGAGTAAATTAAAAGTGTGTGTGATGTGTGGATTAA
ATCTTTGAAACCACACAAACTTCTCAACAAGAATTAATATATTCAATTATTCATCATACA
TTGAGCTTGATTACTTTTCTTATGTTTGAAACTGGTGTCGACATTGCCAAAGATCTGGTG
GTTTTTGATTCTGCGTTTGGCTCTCCATTCATCTGCTTTATAATCAGCGACCACATATCA
AACAGAATCATGCTACTCCACATTCACAAGGCATTTCAAGTATGAAGTCACTTACGAAAT
AACAAAATAACAAAAAGAAACATAATCAATCATTGGGGCTCAAGCTTTGAAGCTGGAAAA
TTCCCACTGTACTGAAACGATTCACTGCGGACAAATTGACGCTTAAACAACTTAATAACA
CATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAAC
AGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTT
TGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 25-K117944-SALK_118908C-R210
GenBank Accession LN485015 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K117944-SALK_118908C-R210
TTTGTCCGCAGTGAATCGTTTCGTACAGTGGGAATTTTCCAGCTTCAAAGCTTGAGCCCC
AATGATTGATTATGTTTCTTTTTGTTATTTTGTTATTTCGTAAGTGACTTCATACTTGAA
ATGCCTTGTGAATGTGGAGTAGCATGATTCTGTTTGATATGTGGTCGCTGATTATAAAGC
AGATGAATGGAGAGCCAAACGCAGAATCAAAAACCACCAGATCTTTGGCAATGTCGACAC
CAGTTTCAAACATAAGAAAAGTAATCAAGCTCAATGTATGATGAATAATTGAATATATTA
ATTCTTGTTGAGAAGTTTGTGTGGTTTCAAAGATTTAATCCACACATCACACACACTTTT
AATTTACTCCAAACCTTTCACATTTATGCATGAGATTATATTACAAATTTATATTACAAA
TAATTTGTAGTACGTAAGTAAAATTAGTATTAACTAATAAGAAAAAAAAGTAATAAAGTT
ATTGGGTTTGCAACCTAACACATCAACGATCCCATCGAACAACAACAGTTACATGACCCA
GGGTGAATTTATGGTTGTCGCTCCATCTGA

Last Updated on 06.12.2018 16:11