Sequences from confirmation amplicon


Confirmation sequences for line SALK_122458 and hit At5g65750 are shown.
Line ID SALK_122458
Locus At5g65750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26307915
Primers used for PCR 45kk - LBb3, show primer details
   
Sequence Name 78-K113914-SALK_122458C-45kk
GenBank Accession LN486190 [GenBank]
Sequencing primer gene specific primer, 45kk: 5'-TCAGGTCTACTATGAGCTTGACGA-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K113914-SALK_122458C-45kk
AGAGTAGAGCAGCTTTGCCCATTTCCATATGATCTCATCCAAAGAGAACTAAAGCGATAT
CCAAGTAGGCGTCGAAAACTCAAGTTTGTGTTCAATAGTTTTGGTTGATTATGGAATTCT
TTGAAACTTTTGTTCTTGTGTTTAACAGATGCAGAGATCGTGTGGTGTCAAGAAGAGCCG
ATGAACATGGGAGGATACCAATACATAGCCCTAAGGCTTTGCACCGCGATGAAAGCACTG
CAAAGAGGAAACTTCAACGACATCAAATACGTTGGTCGTCTTCCCTCAGCTGCTACAGCC
ACAGGATTTTACCAGCTTCATGTTAAGGAGCAGACTGATCTTGTGAAGAAAGCTCTTCAA
CCTGACCCTTAATAACACATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTT
AATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCA
CCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAA
AATCCTGTTTGATGGTGGTTCC
   
Sequence Name 67-K113913-SALK_122458C-R210
GenBank Accession LN485933 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K113913-SALK_122458C-R210
GGGTCGAAGAGCTTTTCTTCACAAGATTCAGTCAGCTTCCTTAACATGAAGCTGGTAAAA
TCCCTGTGGCTGTAGCAGCTGAGGGAAGACGACCAACGTATTTGATGTCGTTGAAGTTTC
CTCTTTGCAGTGCTTTCATCGCGGTGCAAAGCCTTAGGGCTATGTATTGGTATCCTCCCA
TGTTCATCGGCTCTTCTTGACACCACACGATCTCTGCATCTGTTAAACACAAGAACAAAA
GTTTCAAAGAATTCCATAATCAACCAAAACTATTGAACACAAACTTGAGTTTTCGACGCC
TACTTGGATATCGCTTTAGTTCTCTTTGGATGAGATCATATGGAAATGGGCAAAGCTGCT
CTACTCTGCAAATGGCTACATCCTTTGTTTCAGACTTCTTTCGCTCTTCGTCAAGCTCAT
A

Last Updated on 06.12.2018 16:11