Sequences from confirmation amplicon


Confirmation sequences for line SALK_124719c and hit At5g61460 are shown.
Line ID SALK_124719c
Locus At5g61460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24714789
Primers used for PCR 21kk - LBb3, show primer details
   
Sequence Name 76-K113949-SALK_124719C-21kk
GenBank Accession LN486154 [GenBank]
Sequencing primer gene specific primer, 21kk: 5'-TGGAGCTTTCTTTGACGTTAATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K113949-SALK_124719C-21kk
TATCAGCGGACACATCAAAGTCAGTTATGAAAATAAAACTTTGTCCATAGAGGTAATTGA
CACTGCCATGGTACGGTTTTTGCTACTAGCGTGCCCATATTATTACGTCATCTGATTGAT
ATCGTTCTTTATGACAGGTTAAAATGCCTCAAGACGCAACAAGCAATGTCGTTCGAGACA
CCAAAGGTCTTTCAGGTACTGCATCCTTCCACACTCTTAAAAATCATACATCTGATTCAT
TGCCATATAAAGACATTTCCTATGTGTAACGCTCTTCTCATTATACTAGGCGGAGAACGT
TCTTTCTCAACTTTATGTTTTGCACTAGCTCTTCACGAGATGACAGAAGCCCCGTTTCGA
GCAATGGATGAGTTTGATGTGTTTATGGTATTATGTCCTTTTAAGAATTCTCTCTTTTAC
GAGCTTTACGTTGGGATGAGACTAACATTTTTAACTTTCTGATTCTGAAATATAGGATGC
AGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACG
TTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCC
CTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAG
GCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 65-K113948-SALK_124719C-R210
GenBank Accession LN485876 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K113948-SALK_124719C-R210
CCACAATATATCCTGCATCCTATATTTCAGAATCAGAAAGTTAAAAATGTTAGTCTCATC
CCAACGTAAAGCTCGTAAAAGAGAGAATTCTTAAAAGGACATAATACCATAAACACATCA
AACTCATCCATTGCTCGAAACGGGGCTTCTGTCATCTCGTGAAGAGCTAGTGCAAAACAT
AAAGTTGAGAAAGAACGTTCTCCGCCTAGTATAATGAGAAGAGCGTTACACATAGGAAAT
GTCTTTATATGGCAATGAATCAGATGTATGATTTTTAAGAGTGTGGAAGGATGCAGTACC
TGAAAGACCTTTGGTGTCTCGAACGACATTGCTTGTTGCGTCTTGAGGCATTTTAACCTG
TCATAAAGAACGATATCAATCAGATGACGTAATAATATGGGCACGCTAGTAGCAAAAACC
GTACCATGGCAGTGTCAATTACCTCTATGGACAAAGTTTTATTTTCATAACTGACTTTGA
TGTGTCCGCTGATACCTTTCTTTCCCAAGTGAGCGTTGAATCTGCTTAGAAGATTAACGT
CAAAAAAAGCTCC

Last Updated on 06.12.2018 16:11