Sequences from confirmation amplicon


Confirmation sequences for line SALK_125066c and hit At1g30630 are shown.
Line ID SALK_125066c
Locus At1g30630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10858779
Primers used for PCR 03du - LBb3, show primer details
   
Sequence Name 71-K110715-SALK_125066C-03du
GenBank Accession LN486026 [GenBank]
Sequencing primer gene specific primer, 03du: 5'-AACAGATTGATGAAGACCACACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K110715-SALK_125066C-03du
TCTGGCATAGTATGATATGAATATCCATTTCTTTTTTCCATATAGTGTAGAGAAGTTTGT
TAATGTGTTTCTTAATATGAAATTGTTCTTTATGATCTCAGGGTGGTTCCAAGATACAGG
AAGCTTATCTAATCTTCCAGGATTTCTCTGAGAAGTACCCAATGACAAGCTTGATCTTGA
ACGGCAAAGCAGTTTGCTGCATGCATATGGGTAATTTTGAAGAAGCTGAGACTCTACTAC
TTGAAGCACTCAACAAGGTGAGTGATGAACCTACTCTTTCTTTCCTTAAATCTGTATCGA
CACAACCCATTCTCAATATCATGTCATCGGAAATGCTCACTCGATTTAAATCTATGAGGA
TACTTGAAAACTCCTTACACCAAATCTAAACTTTTCAACTATTCCGTTTATTATAAAATG
GACAAGTTTGAAATCATAGATGTTTATTCTCATTGCAGGATGCTAAAGACCCAGAAACTC
TTGCAAACCACGATATATTGACGCTTAACCAGCATATATTGTGGTGTAAACAAATTGACG
CTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTT
CACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAG
CAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAA
TCGGGCAAA
   
Sequence Name 55-K110714-SALK_125066C-R210
GenBank Accession LN485622 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K110714-SALK_125066C-R210
GTCATTTGTTTCACCACAATATATGCTGGTTAAGCGTCAATATATCGTGGTTTGCAAGAG
TTTCTGGGTCTTTAGCATCCTGCAATGAGAATAAACATCTATGATTTCAAACTTGTCCAT
TTTATAATAAACGGAATAGTTGAAAAGTTTAGATTTGGTGTAAGGAGTTTTCAAGTATCC
TCATAGATTTAAATCGAGTGAGCATTTCCGATGACATGATATTGAGAATGGGTTGTGTCG
ATACAGATTTAAGGAAAGAAAGAGTAGGTTCATCACTCACCTTGTTGAGTGCTTCAAGTA
GTAGAGTCTCAGCTTCTTCAAAATTACCCATATGCATGCAGCAAACTGCTTTGCCGTTCA
AGATCAAGCTTGTCATTGGGTACTTCTCAGAGAAATCCTGGAAGATTAGATAAGCTTCCT
GTATCTTGGAACCACCCTGAGATCATAAAGAACAATTTCATATTAAGAAACACATTAACA
AACTTCTCTACACTATATGGAAAAAAGAAATGGATATTCATATCATACTACTGCCAGATT
CAACCACGCGCTCGCGAGCTGAGTGAGTGTGTGGTCTTCATCAATCTGT

Last Updated on 06.12.2018 16:11