Sequences from confirmation amplicon


Confirmation sequences for line SALK_127086c and hit At5g09690 are shown.
Line ID SALK_127086c
Locus At5g09690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3001812
Primers used for PCR 47kq - LBb3, show primer details
   
Sequence Name 30-K114624-SALK_127086C-47kq
GenBank Accession LN485132 [GenBank]
Sequencing primer gene specific primer, 47kq: 5'-TGCAAGGACAACAGAACTAGAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K114624-SALK_127086C-47kq
CTCAGCAATTATTGTCATCACTGATCTAATTCCAACTTCATTTAACTCCATTCAGTCGAT
GAGAACCGTTTCATTTTGTAGATAAGTAGCCGTAACTTGGAAAGAGTTCGTAAATTGAAG
AGTGCCATGACCCGTTTGACAGCTCGGGTCCAAAAGGTGAGTATGTTTCACTGGTAAACA
GAGTCTATGTTTAGAACTTGTGTTGCCACATATACTATGAGTATGAAGCTTCCTCACATA
TGTCAAGTGAACATTCAAGATATACGATAGTGGAACAGAGAAGAATTAATCTCTCTCGTC
GCTTTTTTTTCAGGTAAGGGATGAGCTTGAACAGTTGTTGGATGATGATGGTGATATGGC
TGATCTCTACCTTACAAGGAAGCTTGTTGGTGCATCTTCATCTGTCAGCGTTTCAGATGA
ACCCATTTGGTATCCTACTTCCCCAACAGGATATATTGTGGTGTAAACAAATTGACGCTT
AGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCAC
CAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAA
GCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 14-K114623-SALK_127086C-R210
GenBank Accession LN484674 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K114623-SALK_127086C-R210
ACCACAATATATCCTGTTGGGGAAGTAGGATACCAAATGGGTTCATCTGAAACGCTGACA
GATGAAGATGCACCAACAAGCTTCCTTGTAAGGTAGAGATCAGCCATATCACCATCATCA
TCCAACAACTGTTCAAGCTCATCCCTTACCTGAAAAAAAAGCGACGAGAGAGATTAATTC
TTCTCTGTTCCACTATCGTATATCTTGAATGTTCACTTGACATATGTGAGGAAGCTTCAT
ACTCATAGTATATGTGGCAACACAAGTTCTAAACATAGACTCTGTTTACCAGTGAAACAT
ACTCACCTTTTGGACCCGAGCTGTCAAACGGGTCATGGCACTCTTCAATTTACGAACTCT
TTCCAAGTTACGGCTACTTATCTACAAAATGAAACGGTTCTCATCGACTGAATGGAGTTA
AATGAAGTTGGAATTAGATCAGTGATGACAATAATTGCTGAGTTAGCTACCTTCAAGGTA
AGTTCATCCAAAGCAGGATAAGCAAACTTCTCTAGTTCTGTGTTCCTTGC

Last Updated on 06.12.2018 16:11