Sequences from confirmation amplicon


Confirmation sequences for line SALK_131274c and hit At3g05240 are shown.
Line ID SALK_131274c
Locus At3g05240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1493787
Primers used for PCR 31hd - LBb3, show primer details
   
Sequence Name 43-K112623-SALK_131274C-31hd
GenBank Accession LN485354 [GenBank]
Sequencing primer gene specific primer, 31hd: 5'-TTTTGTTTTCGTAGCAGCAGAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K112623-SALK_131274C-31hd
CTGAATGCAAGGCTTCTTCTTAGGATTGACTTAAGATTGGAACTCTTCACCAATGGATAT
TGACATTTCGTTTGCAGGTTTCTCTATGGGATGCCATTATTCCTGCCAAGGAACATGCCA
AATTCCGGATTCAAGTCTCAAACAAGTACCGTTTCATCGATCAGGTACAGTAAACAAGCT
GGACAAGTCTTCTGCGTTTTTACTTCGTCAGAAACGGAAACTGATCATCATTTGGGATTT
TTCAGGGACAGAATCTTCGGGGCAAAGACTTCAACTTGACATTACACTGGCATGTCATGC
CCAAGACCGGGAAGATGTTTGCTGACAAGATAGTCTTGCCTGGTTACAGTTTACCAGATG
CATACAGATGATGAAGATTGAATAAAACATCAAACCTAGTCCAAGACTTTTTGTATTTCA
GTTTTCTGAAAAGTTTTAGACTTTCTTTTATTCCAAAGAAAACTAATTCAAAAGTACTAT
TGTTCTCTTAGAATCAAACAAAACTATTCTTTTTAGTGATCTTAATTAGTTGTTAATGAG
GTGAAAATCACGACCTCTTAATTTAAAATAAGGTTTATAACGAGGTCAAGTAGGACCCTG
AATTAGAACATTGTTTCAACAGAACTATGACCTAAAACTTTATCTACCCTTTTGCTCTTC
ATGGATTCTCTAATTAGCTTAACATCAGCCCATCGGCCAGCTTTGGTGTAAACAAATTGA
CGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTT
TTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGC
AGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
   
Sequence Name 19-K112622-SALK_131274C-R210
GenBank Accession LN484816 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K112622-SALK_131274C-R210
GTCATTTGTTACACCAAAGCTGGCCGATGGGCTGATGTTAAGCTAATTAGAGAATCCATG
AAGAGCAAAAGGGTAGATAAAGTTTTAGGTCATAGTTCTGTTGAAACAATGTTCTAATTC
AGGGTCCTACTTGACCTCGTTATAAACCTTATTTTAAATTAAGAGGTCGTGATTTTCACC
TCATTAACAACTAATTAAGATCACTAAAAAGAATAGTTTTGTTTGATTCTAAGAGAACAA
TAGTACTTTTGAATTAGTTTTCTTTGGAATAAAAGAAAGTCTAAAACTTTTCAGAAAACT
GAAATACAAAAAGTCTTGGACTAGGTTTGATGTTTTATTCAATCTTCATCATCTGTATGC
ATCTGGTAAACTGTAACCAGGCAAGACTATCTTGTCAGCAAACATCTTCCCGGTCTTGGG
CATGACATGCCAGTGTAATGTCAAGTTGAAGTCTTTGCCCCGAAGATTCTGTCCCTGAAA
AATCCCAAATGATGATCAGTTTCCGTTTCTGACGAAGTAAAAACGCAGAAGACTTGTCCA
GCTTGTTTACTGTACCTGATCGATGAAACGGTACTTGTTTGAGACTTGAATCCGGAATTT
GGCATGTTCCTTGGCAGGAATAATGGCATCCCATAGAGAAACCTGCAAACGAAATGTCAA
TATCCATTGGTGAAGAGTTCCAATCTTAAGTCAATCCTAAGAAGAAGCCTTTGCATTCAG
CTGTCATACCTGATTCAGGGAATTCTTTGGGGTTTCATACTCTGCTGCTACAAAAACAAA
AA

Last Updated on 06.12.2018 16:11