Sequences from confirmation amplicon


Confirmation sequences for line SALK_141443c and hit At1g20910 are shown.
Line ID SALK_141443c
Locus At1g20910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7279872
Primers used for PCR 56dl - LBb3, show primer details
   
Sequence Name 54-K110195-SALK_141443C-56dl
GenBank Accession LN485601 [GenBank]
Sequencing primer gene specific primer, 56dl: 5'-AATCACAGTACATGAAACCTTCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K110195-SALK_141443C-56dl
TCTTCTAATTTACGGTGCTAGATCGATCTTCATCCAAACTTTCCATATCTACCATTATTT
CTATATATTATTCAGTAGTGTCTAAAATCCAAAAGCCATTTTTTTAGGGGAAATTCATAC
CTCTTTCTCGACACTGGAAGACAGAATCAATGTTGAACCAGGAAGGTTAAGCTCACCATT
ATTCCTTAAACACTTTTCGTACTCCAAAAGTGCCTGCAATTGAAGCAAAGATATAGATCA
TATGTACCTGTATCTATATGATAAGATAGAAAGAAAACAAGCAGTGCATTCAAACTTCGA
AGTGACAAAAGAATAGTCCAAAATAAAGAGCTACCTCAGCAAGACAAAGAATTAAACTAA
CTATTATAACACACCATACTAAGAAGAAGCACAAGGAGAATTGTCTGAAGTGGATAAGTA
AAAATGAAGACAGGATAAATATACGATACTTATGCACAAAAACAAACTCACCTTCTCATA
GAAATTGCGGAATGTGTAGGAGACTGTTGTGCATGTCCTGAATAAAGATAGTTTAACCAA
ATGTGGAAAAAGATTAGCATTATGAAAAATTGGTAAAGACTAACGGAGAGTCTAAGGAAA
ATATCAGCCATACTTGGGAGGATTGAAGGATTCTCCAACTTGCCGCCACAGCTTATTTGT
GGTGACCTACATTACCATGAAAAAACAAAGGAATCTTAAAGACCATAAGTCCAGCAGAAG
ATTAAGAAAACATTGAATATATAATTCCAAATATAGCTAACAACAGTAAAATAAATCAGT
TTCCCAAGAAAATATCGAACATTCTTTAATGTTTGATATGTTCGCTTAAAGTCATAAA
   
Sequence Name 46-K110194-SALK_141443C-R210
GenBank Accession LN485411 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K110194-SALK_141443C-R210
TTGGTTCATTTTATTGTTTGAATTGTGGATGGCATATGAACTGTATATAGGGTATAACTA
TTGAATCCGTAAGCTAATTTATGATAAGAGTATCTATAACGATGTGGCTTTCAGAGAAGA
TTTATGACTTTAAGCGAACATATCAAACATTAAAGAATGTTCGATATTTTCTTGGGAAAC
TGATTTATTTTACTGTTGTTAGCTATATTTGGAATTATATATTCAATGTTTTCTTAATCT
TCTGCTGGACTTATGGTCTTTAAGATTCCTTTGTTTTTTCATGGTAATGTAGGTCACCAC
AAATAAGCTGTGGCGGCAAGTTGGAGAATCCTTCAATCCTCCCAAGTATGGCTGATATTT
TCCTTAGACTCTCCGTTAGTCTTTACCAATTTTTCATAATGCTAATCTTTTTCCACATTT
GGTTAAACTATCTTTATTCAGGACATGCACAACAGTCTCCTACACATTCCGCAATTTCTA
TGAGAAGGTGAGTTTGTTTTTGTGCATAAGTATCGTATATTTATCCTGTCTTCATTTTTA
CTTATCCACTTCAGACAATTCTCCTTGTGCTTCTTCTTAGTATGGTGTGTTATAATAGTT
AGTTTAATTCTTTGTCTTGCTGAGGTAGCTCTTTATTTTGGACTATTCTTTTGTCACTTC
GAAGTTTGAATGCACTGCTTGTTTTCTTTCTATCTTATCATATAGATACAGGTACATATG
ATCTATATCTTTGCTTCAATTGCAGGCACTTTTGGAGTACGAAAAGTGTTTAAGGAATAA
TGGTGAGCTTAACCTTCCTGGTTCAACATTGATTCTGTCTTCCAGTGTCGAGAAAGAGGT
ATGAATTTCCCCTAAAAAAATGGCTTTTGGATTTTAGACACTACTGAAATATATATAGAA
ATAATGGTAGATATGGAAAATTTGGATGAAGATCGATCTAGCCCGTAAAATTAAGGAAGA
TATCTACTT

Last Updated on 06.12.2018 16:11