Sequences from confirmation amplicon


Confirmation sequences for line SALK_150463c and hit At3g54960 are shown.
Line ID SALK_150463c
Locus At3g54960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20366040
Primers used for PCR 35dh - LBb3, show primer details
   
Sequence Name 56-K110195-SALK_150463C-35dh
GenBank Accession LN485641 [GenBank]
Sequencing primer gene specific primer, 35dh: 5'-AAGCTTGATGAGAGAGGCATAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K110195-SALK_150463C-35dh
GAGGTATGTATTCGAGGAGTCTTACCAAAACGGGCAAGTTTCTCTTCCTCCTTTTTCAGT
AAAACTAAGGCAGGACGCTTGACTTGGGTTTCAATTTCAAATAGCTTTGCAATATCAGGG
CTTGCAGTTTGGTAAAAACTGAGATCGTCTTCCAGTCTGGACGCAGCCGCGAGCTCCTCA
CTCTCTGACCCCTGAGCAAGAGAAAATGGTAGCAAAAATAAGATATTAAAAGCAAAATCT
TTCACACTTGTCAATCCAACATCTTAAAACTCTTTGCCACACAAAGTAAAACACACTAGA
CAAGAAGATCTGATATACAAGACCTAATAGCAACCTTACATATACTGCAAGGGAAACCAC
TCGTATCCTTGATATACTAAACACCAGAGGCACTCATTTGCGTAACGATAATAGTTTCAT
TTCTTATCCTTTCTGAAATTCTATGGTAGAGAAGTCCCACAGTAACCAACTTGGTTGACA
ATATGTTTAAAGCTCTGAGAAGTTAAAAAGGAGAACTAAATCCAAGAAAGAACGCAAGAA
ACACCATGGATTTAAAGGATGCATACCACTAATGAGTTGAGAAAACCAAAGACAAGTTTA
GGTTCAGCGGACAAAACACGTTCAGCCTCCTCTTTAGTAGTGATGTTGTGGATGCTAGGA
CTGGCCTTCTTCTTCAGCCAAGTCACAATACCATCCCTGCAATGAGGACACCAGTAATGG
TGAAACGTACTTTTTGACCAATAATTCGTATGGAAAACTAACAAATTGTGGTGTAAACAA
ATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTT
TTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACGCCTGGCCCTGAGAGAG
TTGCAGCAAGCGGGTCACGCTGGTTTG
   
Sequence Name 48-K110194-SALK_150463C-R210
GenBank Accession LN485454 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K110194-SALK_150463C-R210
GCGTCATTTGTTTACCCACAATTTGTTAGTTTTCCATACGAATTATTGGTCAAAAAGTAC
GTTTCACCATTACTGGTGTCCTCATTGCAGGGATGGTATTGTGACTTGGCTGAAGAAGAA
GGCCAGTCCTAGCATCCACAACATCACTACTAAAGAGGAGGCTGAACGTGTTTTGTCCGC
TGAACCTAAACTTGTCTTTGGTTTTCTCAACTCATTAGTGGTATGCATCCTTTAAATCCA
TGGTGTTTCTTGCGTTCTTTCTTGGATTTAGTTCTCCTTTTTAACTTCTCAGAGCTTTAA
ACATATTGTCAACCAAGTTGGTTACTGTGGGACTTCTCTACCATAGAATTTCAGAAAGGA
TAAGAAATGAAACTATTATCGTTACGCAAATGAGTGCCTCTGGTGTTTAGTATATCAAGG
ATACGAGTGGTTTCCCTTGCAGTATATGTAAGGTTGCTATTAGGTCTTGTATATCAGATC
TTCTTGTCTAGTGTGTTTTACTTTGTGTGGCAAAGAGTTTTAAGATGTTGGATTGACAAG
TGTGAAAGATTTTGCTTTTAATATCTTATTTTTGCTACCATTTTCTCTTGCTCAGGGGTC
AGAGAGTGAGGAGCTCGCGGCTGCGTCCAGACTGGAAGACGATCTCAGTTTTTACCAAAC
TGCAAGCCCTGATATTGCAAAGCTATTTGAAATTGAAACCCAAGTCAAGCGTCCTGCCTT
AGTTTTACTGAAAAAGGAGGAAGAGAAACTTGCCCGTTTTGGTAAGACTCCTCGAATACA
TTACCTTCTTTTTTTGTGCTACTTAGATTTCCTCTATGCCTC

Last Updated on 06.12.2018 16:11