SimpleSearch - Line and FST details


Line specific information

 
Line ID 011E08
Vector Used pAC106
Line Availability only available from NASC (N401016)
Segregation Analysis 100:92:68
Confirmed for Hit At5g66710
Parent of DUPLO pair none
Parent of pair(s) 2242, 2692, 97316
Note Although the line exists, the insertion in At2g46510 can not be ordered because the confirmation of this insertion failed.

Gene hit At2g46510

 
Sequence (A. th genome BLAST matches underlined)
>61-K012433-022-011-E08-8409
NTTTGATACCATGTAGATTTCCCGGACTGAAGCACTTACATTGAATTATCCTGAAATTCA
TTGGATTTTCTAAAGGTAGGAAAATTTGCCCAAGATACAAATATGTGACATCGTGGGCTA
AATAAAGTAATGAGTTACTTAAGCCGGATTGCTTTCCTATAAGCAAAGAGAAAAGAAAAG
AGGCTTTTATCTATTTCTATGGGATCCTCCCTATAGTGAGTCGTTNTACTACAGANNNCN
NNNNGGGAGGNTTCCCANNTGTTTCTACCTTCGACAAAGAAAGGGTCCGCGGCGAGAGTA
CCATGAGTAAGATCATTAGGAAGATTCGACAGAGGGCGATCCGAATGAGTTATCTGTGAA
GenBank Accession CT025962 [GenBank]
Graphic View Graphic view of gene At2g46510
Predicted Position of Insertion Chr2:19090978 - go to primer design
BLAST e Value 7e-15
Hit Clone Code (BAC ID) F11C10
Hit Gene Code At2g46510 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation ABA-inducible BHLH-type transcription factor
Insertion Classification TS2TE (3')
Confirmation Status failed, line belongs to a contamination group, please check line 013E08
Other FSTs Supporting this Hit CT025961 [GenBank] CT025962 [GenBank]

Gene hit At5g66710

 
Sequence (A. th genome BLAST matches underlined)
>61-K013044-022-011-E08-35St
NTGACGTAAGGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATACTG
ATTTTTGGAATTTGGATATGGGTTAAAGAACATTTCTTTATCCATGATTATAGGTTCAGG
AGAGTTGTCCCTGTGTCTGTGAAGATATTCCAGCCTAAAAGAACATCTGCTTTAAGCATT
GAGCAGAGGAAGAAGTTTCAGAGAGAGGTTTTGCTGCTTTCCAAGTTTAGACATGAAAAC
ATTGTGCGGGTAATATTGGAGCCTTTGATGTTTTGTTTGTTTTCATATGTTAGCAGCCTT
ATCTCGTTGGTCGTCGATGGTATAATATCCCTAATGGGATCCTTTTGATGACAGTTCATT
GGGGCGTGCATAGAACCAAAGTTGATGATAATTACTGAACTCATGGAAGGCAATACTCTT
CAGAAGTTTATGTTGAGTGTTCGTCCAAAGCCTCTTGATCTGAAGCTCTCCATTAGCTTT
GCGTTGGATATTGCTCGGGGAATGGAGTTCTTGAATGCAAATGGCATTATTCACCGAGAT
TTGAAACCAAGTAAGTTAAACTGAGACATCACGAAAAGAGGAATCAAGATTTGTGAATTG
AAGAAGATCGGTTTTTGTGCAGGTAATATGCTCTTAACAGGTGATCAGGAACCTGCTAAG
TTGGCTGACTTTGGACTTGCTATGGGATCCTCCCTATAGTGAGTCGT
GenBank Accession CR356248 [GenBank]
Graphic View Graphic view of gene At5g66710
Predicted Position of Insertion Chr5:26637229 - go to primer design
BLAST e Value 0.0
Hit Clone Code (BAC ID) MSN2
Hit Gene Code At5g66710 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation Protein kinase superfamily protein
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details
Other FSTs Supporting this Hit CR356248 [GenBank]


Last Updated on 31.08.2016 09:40