SimpleSearch - Line and FST details


Line specific information

 
Line ID 054H07
Vector Used pAC161
Line Availability only available from NASC (N405179)
Segregation Analysis 100:98:94
Confirmed for Hit At2g17370 At2g28030
Parent of DUPLO pair none
Parent of pair(s) 9564, 97003

Gene hit F24D13

 
Sequence (A. th genome BLAST matches underlined)
>56-K012301-022-054-H07-8409
ATCCATGAAGACTTCATCTTTTTCGTGTATTAATACCCATCATGCACTAGAAGTCTAGAA
CATAAAATCCTAGGGTTTCTATAATAAGCACACTTTTGCTGAGATTTTCAACTACTAATC
ACCATTTCATACAAGAGTCCTAACTCAAGTCTAACAACACTGAAATAACATTTTAACGGA
CTGTTTTCACGATAGAAGATATATAAAATCACTGAGTATTGGACCATTTTATTGAGTTTT
TTTTTCTAATTAAGACTAATATTCGCCGAAGTAATTTAAACTGTTCGTTTTGCAAATTTG
AGGCTATGGGATCCTCTATTAGAANGAGANNNNAGNCCCAAACTACCAATGGCTGAGTAC
AAAACTTGAAGTGACGTAAAAAGCACAGTAGTCCATCTATTAGAATAATGGCGGTATTGT
GATGCTCCTATGACGCTGATGATTGAACTAGCTACGAAGAAGATGACTAACAGCCGTTGC
TGGATCAATATCATCGCACAAGCGGTTTAGGTCGTCAAGCTACTCGTCGGCGAATAGATA
GCGTGAAATCGCCGTGTACATACTCAGCGTTGTTCTATGGGAGCCTACTATTTNGGGGGG
AATTTCGAATAATATCTTTGGGTGTTCCAGACGCAATGCAATGGGTCTTGATTAATTCCT
GCACGTATTTATCAAGGGTAAAACCTTTTGTCCGAATTGGTAAGCTTTAAATTTTTGGGA
ACTTACGTCCATTTTTGTTATTGCCATTACGTCCTTTAAGCTGGGGGACTTTTAACATTT
TGGTACCACTAAGTTTTAATTTTCTCTCTAAGGTTTAAACTTCTTTTAAAATGACTTTTT
GenBank Accession AL754587 [GenBank]
Graphic View Graphic view of sequence AL754587 of line 054H07
Predicted Position of Insertion Chr2:11967745 - go to primer design
BLAST e Value 1e-130
Hit Clone Code (BAC ID) F24D13
Insertion Classification Genome Hit
Confirmation Status unknown
Other FSTs Supporting this Hit AL754591 [GenBank]

Gene hit At2g17370

 
Sequence (A. th genome BLAST matches underlined)
>56-K012480-022-054-H07-8409
TGATCCATGTAGATTTCCCGGCTTCGTGTATTACTACAGCATCATGCACTAGAAGTCTAG
AACATAAAATCCTATGGGATCCTCCCTATAGTGACAAAAACCATAACCCTAAATAATCAT
CCCAAAAGTGGAAATAATAAAAGAAGCAGTTAGATTGTGACGGAGGAGACAAAATCATCA
TCTCTGTTCTTCTTCTTCTCCTCTCTCACATACGATGATGACCCAACAACCTTGACGTTG
ATTTCGATGTCACACACAACACTATAAATAACACTCTTCACCTATTTTTGTCATCTCACA
CTTTGGACTTTGGAGCCCAAAATAATCTCATAACCATTAAAAACAAATCTCCTCCTTCTC
TCCGGCGTCAATGGAGGATCTCCGTCGTAGATTTCCGACTAAAAAGAACGGAGAAGAAAT
CTCTAATGTCGCCGTCGATCCTCCTCTTCGTAAAGCCTCTGACGCGCTTCCTCTTCCGTT
ATACCTAACCAACACTTTTTTCCTCTCGCTCTTCTTCGCTACTGTTTATTTTCTTCTTAG
CCGATGGCGTGAGAAGATCCGTAACTCGACGCCTCTTCACGTCGTTGACTTATCTGAGAT
CTGTGCTCTAATCGGTTTCGTTGCTTCGTTTATCTACCTCCTTGGTTTCTGTGGGATGGA
TCTCATTTTCCGATCTTCTTCCGATGATGATNGTTGGGGTAACGATGGGATGATTCCATG
TAATCAATCCCTA
GenBank Accession AL754592 [GenBank]
Graphic View Graphic view of gene At2g17370
Predicted Position of Insertion Chr2:7549695 - go to primer design
BLAST e Value 0.0
Hit Clone Code (BAC ID) F5J6
Hit Gene Code At2g17370 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation 3-hydroxy-3-methylglutaryl-CoA reductase 2
Insertion Classification Promoter
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details

Gene hit At2g28030

 
Sequence (A. th genome BLAST matches underlined)
>56-K012489-022-054-H07-35St
NACTTGACGAAGGGATGACGCTTANTCCACTATCCTTCGCAGACCCTTCCTCTATATAAG
GAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCT
TCGGTTTAAACTATCGCCGTCATATACACTCATATACACTCATGGTTGGTCTAAGCTGGG
GACCTTCATCGCTCATCACTCAGATGGGCGGTGAGTACCCAGGTTTGATGTCTTACTGTT
TTGCTAGTCAAGGAACTAGTAAGATCAATTTTGGAACAAATGCTATTGTTGCAGGAGATG
GGGTTGTATCAACCACTATGTTTCTCACGACGGCGAAACCAGGTTTATATTACCTAAATC
TAGACGCGGTCAGCGTTGGGGACACCCATGTTGAGACAATGGGGACAACGTTTCATGCGT
TAGAAGGGAACATAATTATAGACTCTGGAACCACTCTAACCTACTTTCCTGTGAGCTACT
GCAACCTATGGGATCTCTTTTAANTNGAGANNNNNNNNNTNNTGNNNNTTTATNANNCTC
TATATTTCCGTNACATGATGATGTGGGGCGAGTTGTTGGATTTTGGNTTTTAATTNGGGT
TTTGTGGGGGGAACTTCACGAAAATATTCCAAACGCAGCAAGATATCGCGGTGCATCTTC
GGTCTTGCCTGGGCCAATCGCCGCCGACGCCGGTTGATGTGGCCGCCCGGCCCCGATCAT
ATTGTCGCTCAAGATCCGGGCGTTGGTGCTTGTCCGCCGTTGCTGTCGTAATGATATTCG
GGACTTTCACCCGCCTGTTCCGCAGAGGGGCCGGGCCTTCAACTTAAACCCCGAACCGTT
GGAAATTGCGGG
GenBank Accession AL754593 [GenBank]
Graphic View Graphic view of gene At2g28030
Predicted Position of Insertion Chr2:11934870 - go to primer design
BLAST e Value 0.0
Hit Clone Code (BAC ID) T1E2
Hit Gene Code At2g28030 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation Eukaryotic aspartyl protease family protein
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details


Last Updated on 07.07.2016 14:03