SimpleSearch - Line and FST details
Line specific information
| Line ID | 224B12 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N421432) |
| Segregation Analysis | 100:91:81 |
| Confirmed for Hit | FCAALL |
| Parent of DUPLO pair | none |
| Parent of pair(s) | none |
| Note |
Gene hit At3g50890
| Sequence (A. th genome BLAST matches underlined) | >11-K020163-022-224-B12t-8409 ACTGTTTGTATGATTTCCAGGTGGTGGTATCTATTTTTTCTAGAGAGAGGGGTAAGCTAA GGTGTGCTATGGATTCTTAGATATAGTGAGATCTGATGATTAAATTTGATATATACTGAA TGGGAGGCTGAGTAAAGTGAGAGTATGGGATGTTCCAAAAAAGAGGGAAATACGTGTGTT TTTATTAGTTAAATGGACAAGTATGGCTAAAACGTTTAGCTGGAATAGCTCAGGTGGTTA GAGGGTGTGGCTGTTAACAAGAAGGTTGGAGGTTCGACCGGTATTCTTATGGGATGATCG AACAAAAAGAGTGGGGGGTGATCAGAGGTTTTTTTTTTTTTTCCCCCCCCCCCCTTGAAA TGAGGATTGGACGAAAAAAAAAGGAGGGGGGGGGGGGGTGTATGAATGGTGATGCTTTAT GAGGTGTGTCGTTTGGGGGTTGTTAGGGTCTGGCGCAGAGCGGTGATATGTGGCGAGGCA GGGTGTTGTGGGATGTAGAAGACCCGAAGTTGAGGTGGCGAGGGGGCTACTTTATTTTTA TGACTGAGTGATGTGGCGGGGGTGAGGAGTGGTTTGGATGGCTTGTTTGGTTGAGTTTGT GTGCGAAGTTGTCAGCGGCGCGGGTGTGATGCATGCAATGGAGACAGCGAAGGGGGTAGA GAGGAATAGAGGGCGAGGGTAGTTGTGGATGATGTTAGTTAGGAGATAGGGTTGAGTGCG TTGGTGGTGCTCCGTGGGCGAGGGTGATTGTGTGTGGGTACGTAGGCGGATATTGTGCGG GTAAGTGTAGTGATGTGTCTGTGTCTGTTGATATGGTAGGGGGGTAGGGCGCATAGGGAG GATTGATGTGTTGCGGTAGGGTGTCGGAGAGTGTTATCGTAGTGCATGTCGTGGGGGTAG CAGATGGGGTGGCGGGGTTGCGGGGGTCCGGTGTTGTAGTTGGTAGTTTCTAATTGTGTA GCGTCGATGGCTCTGTGTGTTATAGTAATGAATTGTATATTTGAGAGGGGTGGAGAATGG AGGTGATGA |
| GenBank Accession | FR806061 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:18917115 - go to primer design |
| BLAST e Value | 1e-20 |
| Hit Clone Code (BAC ID) | F18B3 |
| Hit Gene Code | At3g50890 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | homeobox protein 28 |
| Insertion Classification | CDSi |
| Confirmation Status | failed, line belongs to a contamination group, please check line 326G12 |
Gene hit FCAALL
| Sequence (A. th genome BLAST matches underlined) | >90-K014242-022-224-B12-8409 ATTGATCCATGTAGATTNTCCCGTACATGAANGCCTTTACAGGNTTAATATTAATACATC TTAAATCTCATTATTAATTTAGCAGTATACGCCTCTACACTATTTCGAAAATCGGAAGCT CCAGCCTCCAGGGACTACTTCAGCTATGGTTTCAGTCGAGACGCAACTCCTATGGGATCC TCCCTATAGTGAGANNNNNNNNNNNNGNCNCCCNNNNACNNTNGNNNCNACTNNNNNCTA NNATCTACTGCCCTGCGAAAATGCCTACACAAACANAAAAAAACAAATGATCTCAAGTTA TATCCACATGTAGCAACTGGTGGATCTACTCGAAAATAGATATTTCAAACTTCAATGAAA CGATGGCCAAAGCATATGTCAGAACTTTAAAAGACATATTATATTATAATTGTAGATAAA ATTCTGTCTTTTTCTAACCAATATATTTGCCTAAAAATTGGTTCAAAACCCAACAGAACA AATTAATTTAAATATCGATTTCGGGCTGCTCCAAGTACGTATTATTTTAATCTGGACGAA CTTGGACGATTTAGGAAAAACGAG |
| GenBank Accession | AL768059 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr4:9422527 - go to primer design |
| BLAST e Value | 2e-129 |
| Hit Clone Code (BAC ID) | FCAALL |
| Insertion Classification | Genome Hit |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
|
Last Updated on 10.06.2021 13:37 |
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