The GABI-Kat SimpleSeach tool offers information about availability of lines/seed. For the lines that have been confirmed (by PCR in the T2 generation), we have produced T3 seed. These T3 seed have been transferred to NASC and are available from the stock centres as "T3 sets". In these cases, a link to the NASC catalog for ordering is provided. Example: 001H07 => N400091

About 1.700 lines with predicted CDSi hits have already been transferred to NASC as (not confirmed) T2-stocks. Example: 127E03 => N2108305

A total of 8.857 lines (not confirmed) are scheduled in addition to be transferred to NASC as T2-stocks. These lines cover insertion alleles with CDSi hits that have a good chance for confirmation. For example, we have filtered out larger "contamination groups" where only one line among several candidates will be positive for the predicted insertion. This transfer has been hit by the COVID-19 pandemic, currently it is not possible to carry out this action. Once the situation improves, the plan will be reactivated. Nevertheless, the database now tells which lines are among these 8.857 lines. Example: 001A10

The GABI-Kat confirmation service will terminate at the end of 2019. After 2019-12-31 it will no longer be possible to order any seed from GABI-Kat. There have been very few requests in the last 3 months, and the administrative overhead for sending out seed and handling the financial aspects of requests has reached a level that can no longer be handeled. Access to about 14,800 lines is possible via NASC or ABRC as T3 sets. We are working together with NASC to make access to T2-stocks possible. However, the germination rate of our seed stocks (almost 20 years old) has gone down to a level that is very problematic for handling in larger numbers. 

Several months ago, we have started to prepare for stopping the GABI-Kat confirmation service at some time in the future. This service is running since more than 15 years. The reason is that the number of requests is declining (although there are still quite some requests for GABI-Kat insertion lines). In addition, the administrative overhead for handling the requests is getting crazy.

To allow the Arabidopsis community access to “valuable” GABI-Kat insertion alleles even if the line has not yet been confirmed and donated as T3-set to NASC, we will start donating T2-stocks. Obviously, these lines/insertion alleles are NOT confirmed but are donated on the basis of predictions. However, the in-house confirmation rate at GABI-Kat is about 80% which indicates that there is a good chance to confirm the predicted insertion allele after ordering a T2-stock from NASC. Lines that have been donated to NASC as T2-stocks can not be requested from GABI-Kat. Information about which lines have been donated is available from SimpleSearch. An example is here.

A paper describing new features incorporated into SimpleSearch and the GABI-Kat database has just been published in PCP (see here). The most relevant point was the switch to the Araport11 annotation. 

Batch number 157 containing 99 newly confirmed GABI-Kat lines has just been donated to NASC. We are on the way to reach 15.000 GK lines at NASC; we expect to donate about 75 additional lines by the end of 2016. See here for exact numbers of donated lines.

About 6 weeks ago we submitted some additional FSTs to ENA for getting accession numbers. These have now been provided by ENA and were incorporated into SimpleSearch. An example is the FST 23-K121193-0022-588-G12C-8409 (line 588G12, AccNo LT615065, indicating a hit in At3g47770) which has been obtained by chance while attempting to confirm the insertion prediction in At3g47750. 

Between 2016-08-15 and 2016-08-29 we have made a new database version available, and also new versions od the flat files with the database content. The db version number is "GK release 28" (see here) and includes data from 77,034 lines with genome hits. There are 23,582 genes with hits, and there are 14,235 CDSi regions (of protein-coding genes excluding pseudogenes) with hits. The sequence is still from TAIR9, but the annotation is Araport11 (which is also based on the TAIR9 genome sequence of A. thaliana Col-0).

The information displayed on the GABI-Kat database content statistics page has been updated to Araport11 values. At the same time, the database content regarding insertion site annotations is now also based on Arabort11. GABI-Kat insertions located in genes newly included in Araport11 can be identified, as well insertions that are present in UTRs which are more exactly described in the new A. thaliana genome sequence annotation.