Genotyping and primer details


Genotyping and primer details for 151G05 - At1g03290
Line ID 151G05
Locus At1g03290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:810678 of line 151G05 Chr1:810678
   
Insertion allele amplicon 8474 - 15bk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15bk: 5'-AGAGGTTTGAAGAAGAGTTTGCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:811226
   
wt amplicon (over InsPos) 08cr - 15bk
wt amplicon start Chr1:810105
wt amplicon end Chr1:811226
wt amplicon size 1122 bp
Locus specific primer 15bk: 5'-AGAGGTTTGAAGAAGAGTTTGCTG-3'
2nd locus specific primer 08cr: 5'-ACAAACACTAGCGATGAGTTTCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11