Genotyping and primer details


Genotyping and primer details for 037C06 - At1g17770
Line ID 037C06
Locus At1g17770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6121074 of line 037C06 Chr1:6121074
   
Insertion allele amplicon 8474 - CM19
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CM19: 5'-AACCTTTGAATAATGTTCACTGCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6120609
   
wt amplicon (over InsPos) 34rd - CM19
wt amplicon start Chr1:6120609
wt amplicon end Chr1:6121479
wt amplicon size 871 bp
Locus specific primer CM19: 5'-AACCTTTGAATAATGTTCACTGCC-3'
2nd locus specific primer 34rd: 5'-CTAAGCACATTTCACCCCAGTAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11