Genotyping and primer details


Genotyping and primer details for 588F08 - At1g75640
Line ID 588F08
Locus At1g75640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28406324 of line 588F08 Chr1:28406324
   
Insertion allele amplicon 8474 - 07mg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07mg: 5'-ATCCAAAACCACTAAAGAGGTCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28405991
   
Insertion allele amplicon 8474 - EU10
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU10: 5'-CCTTCGAAATCAACGACTCTTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28405839
   
wt amplicon (over InsPos) NK76 - EU10
wt amplicon start Chr1:28405839
wt amplicon end Chr1:28406940
wt amplicon size 1102 bp
Locus specific primer EU10: 5'-CCTTCGAAATCAACGACTCTTAAC-3'
2nd locus specific primer NK76: 5'-AGACTCAAGCACTCACATCTTTCA-3'
   
wt amplicon (over InsPos) 08mg - 07mg
wt amplicon start Chr1:28405991
wt amplicon end Chr1:28406732
wt amplicon size 742 bp
Locus specific primer 07mg: 5'-ATCCAAAACCACTAAAGAGGTCAA-3'
2nd locus specific primer 08mg: 5'-AAGTCTTCACACCAACGACATAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11