Genotyping and primer details


Genotyping and primer details for SALK_076337c - At2g24570
Line ID SALK_076337c
Locus At2g24570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10439261 of line SALK_076337c Chr2:10439261
   
Insertion allele amplicon LBb3 - 58ew
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 58ew: 5'-ACCGTATCGAACCAAACAATTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10438486
   
wt amplicon (over InsPos) 36gc - 58ew
wt amplicon start Chr2:10438486
wt amplicon end Chr2:10439380
wt amplicon size 895 bp
Locus specific primer 58ew: 5'-ACCGTATCGAACCAAACAATTAAC-3'
2nd locus specific primer 36gc: 5'-CTCCATCTTAAACCTACTCAACCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11