Genotyping and primer details


Genotyping and primer details for 498G08 - At3g15460
Line ID 498G08
Locus At3g15460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5215365 of line 498G08 Chr3:5215365
   
Insertion allele amplicon 8474 - CM41
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CM41: 5'-TGTTGAAATTTACATTGGGTTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5215983
   
wt amplicon (over InsPos) 02jb - CM41
wt amplicon start Chr3:5214890
wt amplicon end Chr3:5215983
wt amplicon size 1094 bp
Locus specific primer CM41: 5'-TGTTGAAATTTACATTGGGTTTGA-3'
2nd locus specific primer 02jb: 5'-AAAACAAACACATGGTCATGGTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11