Genotyping and primer details


Genotyping and primer details for SALK_015394c - At4g13345
Line ID SALK_015394c
Locus At4g13345 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:7767945 of line SALK_015394c Chr4:7767945
   
Insertion allele amplicon LBb3 - 30dr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 30dr: 5'-GAGACTGACAAATCTCCTTGTTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:7767056
   
wt amplicon (over InsPos) 07fi - 30dr
wt amplicon start Chr4:7767056
wt amplicon end Chr4:7768172
wt amplicon size 1117 bp
Locus specific primer 30dr: 5'-GAGACTGACAAATCTCCTTGTTCC-3'
2nd locus specific primer 07fi: 5'-ATCTTTCTGCATCTTTTTGTGCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11