Genotyping and primer details


Genotyping and primer details for SALK_092203c - At1g16320
Line ID SALK_092203c
Locus At1g16320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5581395 of line SALK_092203c Chr1:5581395
   
Insertion allele amplicon LBb3 - 51wm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 51wm: 5'-ATCTATCATCTCCGTCAATCCATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5580970
   
wt amplicon (over InsPos) 35xa - 51wm
wt amplicon start Chr1:5580970
wt amplicon end Chr1:5581969
wt amplicon size 1000 bp
Locus specific primer 51wm: 5'-ATCTATCATCTCCGTCAATCCATC-3'
2nd locus specific primer 35xa: 5'-TGTGACCTTGAGTGTCTATATCGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11