Genotyping and primer details


Genotyping and primer details for SALK_026163 - At1g19250
Line ID SALK_026163
Locus At1g19250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6651326 of line SALK_026163 Chr1:6651326
   
Insertion allele amplicon LBb3 - 39ke
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 39ke: 5'-GTTTGTATAAGCAACGGTCCAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6651037
   
wt amplicon (over InsPos) 14ll - 39ke
wt amplicon start Chr1:6651037
wt amplicon end Chr1:6651692
wt amplicon size 656 bp
Locus specific primer 39ke: 5'-GTTTGTATAAGCAACGGTCCAAAT-3'
2nd locus specific primer 14ll: 5'-ACTCTCTTTTGCCTCCTCTTCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11