Genotyping and primer details


Genotyping and primer details for 332A10 - At1g22460
Line ID 332A10
Locus At1g22460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7930210 of line 332A10 Chr1:7930210
   
Insertion allele amplicon 8474 - NT16
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NT16: 5'-CGTGACCCTTCTCTAACCTTTTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7930658
   
Insertion allele amplicon 8474 - NT17
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NT17: 5'-CTCAAACCTTGGAAGAAGAAGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7929766
   
wt amplicon (over InsPos) 16fi - NT16
wt amplicon start Chr1:7929761
wt amplicon end Chr1:7930658
wt amplicon size 898 bp
Locus specific primer NT16: 5'-CGTGACCCTTCTCTAACCTTTTTA-3'
2nd locus specific primer 16fi: 5'-GAAATCTCAAACCTTGGAAGAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11