Genotyping and primer details


Genotyping and primer details for SALK_093560 - At1g25400
Line ID SALK_093560
Locus At1g25400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8911444 of line SALK_093560 Chr1:8911444
   
Insertion allele amplicon LBb3 - 90by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 90by: 5'-TAAACGACCCGTCGTATTTATCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8912274
   
wt amplicon (over InsPos) 03dg - 90by
wt amplicon start Chr1:8911205
wt amplicon end Chr1:8912274
wt amplicon size 1070 bp
Locus specific primer 90by: 5'-TAAACGACCCGTCGTATTTATCTC-3'
2nd locus specific primer 03dg: 5'-ACATGAAATCCAAAATACACGACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11