Genotyping and primer details


Genotyping and primer details for SALK_107689c - At1g72670
Line ID SALK_107689c
Locus At1g72670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27358163 of line SALK_107689c Chr1:27358163
   
Insertion allele amplicon LBb3 - 23rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 23rb: 5'-TGCATATACCATAGCTCTCTCCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27357589
   
wt amplicon (over InsPos) 34rz - 23rb
wt amplicon start Chr1:27357589
wt amplicon end Chr1:27358585
wt amplicon size 997 bp
Locus specific primer 23rb: 5'-TGCATATACCATAGCTCTCTCCCT-3'
2nd locus specific primer 34rz: 5'-CTCGAAAAGGCTCTCTTCTTTACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11