Genotyping and primer details


Genotyping and primer details for SALK_017105 - At2g23420
Line ID SALK_017105
Locus At2g23420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9973828 of line SALK_017105 Chr2:9973828
   
Insertion allele amplicon LBb3 - 08en
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 08en: 5'-TGTTCTATTTCTGCTTCAACCCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9973347
   
wt amplicon (over InsPos) 27gc - 08en
wt amplicon start Chr2:9973347
wt amplicon end Chr2:9974166
wt amplicon size 820 bp
Locus specific primer 08en: 5'-TGTTCTATTTCTGCTTCAACCCTT-3'
2nd locus specific primer 27gc: 5'-TTAATCCGAGGCTGATTGTTTATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11