Genotyping and primer details


Genotyping and primer details for SALK_120435 - At2g26190
Line ID SALK_120435
Locus At2g26190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11147835 of line SALK_120435 Chr2:11147835
   
Insertion allele amplicon LBb3 - 39et
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 39et: 5'-GAAGAAGAAGCAGAGAAGAAACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11148242
   
wt amplicon (over InsPos) 13gc - 39et
wt amplicon start Chr2:11147329
wt amplicon end Chr2:11148242
wt amplicon size 914 bp
Locus specific primer 39et: 5'-GAAGAAGAAGCAGAGAAGAAACCA-3'
2nd locus specific primer 13gc: 5'-TACGGATTAGCGAAGAAAAGAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11