Genotyping and primer details


Genotyping and primer details for SALK_019429c - At2g34380
Line ID SALK_019429c
Locus At2g34380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14514250 of line SALK_019429c Chr2:14514250
   
Insertion allele amplicon LBb3 - 16ty
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 16ty: 5'-TCTCTGAAATCCAAACCTTGAACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14513936
   
wt amplicon (over InsPos) 30uu - 16ty
wt amplicon start Chr2:14513936
wt amplicon end Chr2:14514598
wt amplicon size 663 bp
Locus specific primer 16ty: 5'-TCTCTGAAATCCAAACCTTGAACT-3'
2nd locus specific primer 30uu: 5'-AAGGTCAAGCATCTTCAAATCTGT-3'
   
Note

Last Updated on 06.12.2018 16:11